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ProteomicsPrimary human cardiac ventricular fibroblasts
Transformed AC16 cells were acquired from Millipore and used at P10-15 and expanded in DMEM/F-12 with 10% FBS (Gibco). Primary human ventricular cardiac fibroblasts were acquired from Promocell and expanded in FGM-3 (Promocell) and used at P3-5. For the experiment, cells were separately placed on tr...
ORGANISM(S): Homo Sapiens (human) 
2023-05-05 | PXD041982 | JPOST Repository
ProteomicsHuman AC16 Cardiac Cells under Doxorubicin Treatment
AC16 acquired from Millipore and cultured in DMEM/F12 with 10% FBS. For the mass spectrometry experiment, the AC16 cells were switched to Fibroblast Growth Medium (FGM-3) with supplements (Promocell) then treated with 0.1 µM doxorubicin for 24 hours, then co-cultured through non-contact transwells w...
ORGANISM(S): Homo Sapiens (human) 
2023-04-22 | PXD041722 | JPOST Repository
ProteomicsSimultaneous proteome localization and turnover analysis of cardiomyocytes under proteasome inhibition
To examine the protein spatial and temporal changes upon carfilzomib-mediated proteasome inhibition in cardiac cells, we produced human iPSC-derived cardiomyocytes using a standard small molecule based protocol. Cardiomyocyte identity was confirmed by morphology, observation of contraction, and the ...
ORGANISM(S): Homo Sapiens (human) 
2023-04-28 | PXD041386 | JPOST Repository
ProteomicsSimultaneous proteome localization and turnover analysis reveals spatiotemporal coordinations of unfolded protein response
The functionality of proteins is dependent on their spatial and temporal distributions, neither of which is directly measured by static protein abundance. Here we report a mass spectrometry-based proteomics workflow and data analysis pipeline, named Simultaneous Proteome Localization and Turnover (...
ORGANISM(S): Homo Sapiens (human) 
2022-12-31 | PXD038054 | JPOST Repository
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