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ProteomicsRefDIC Protein profiling of mouse immune cells
Not available
ORGANISM(S): Mus musculus (Mouse) 
2011-04-15 | PRD000029 | Pride
TranscriptomicsGene expressions of memory B cells and other type of B cells
To obtain insight into the genetic basis of the increase of functional activity of memory B cells over time, we compared the gene expression profiles of day 7 and day 40 NP-specific/IgG1 memory B cells, GC B cells and plasma cells in immunized WT mice and naïve B cells, before and after activation i...
ORGANISM(S): Mus musculus 
2012-09-21 | E-GEOD-11961 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human U927 cells polysomal and cytoplasmic RNA before and after PMA stimulation
Human histomonocytic U937 cells exhibit macrophage-like properties after phorbol ester stimulation. Accumulating evidence demonstrated that remarkable number of transcripts changed in their amount at total RNA levels. However, post-transcriptional regulation has not been fully elucidated in this m...
ORGANISM(S): Homo sapiens 
2008-06-11 | E-GEOD-1783 | biostudies-arrayexpress
TranscriptomicsConditional deletion of Bcl6 in naive B cells
Bcl6 germline deletion causes a prominent inflammatory disease, owing to over-expression of Th2 cytokines, and affects the properties of B cells prior to immunization. Therefore we established the B cell-specific Bcl6 deletion mice and analyze the gene expression of naive B cells under physiological...
ORGANISM(S): Mus musculus 
2012-09-21 | E-GEOD-28737 | biostudies-arrayexpress
TranscriptomicsRegulation of B cell early development by CNOT3
The CCR4-NOT complex, bearing poly(A) deadenylation activity, is a highly conserved regulator that is involved in biological control; however its action mechanisms and physiological targets remain unclear. Using genetic deletion of the CNOT3 subunit of this complex in early B cell progenitors, we sh...
ORGANISM(S): Mus musculus 
2015-07-23 | E-GEOD-64455 | biostudies-arrayexpress
TranscriptomicsHuman PBMNCs: Control vs. Patients with Kawasaki disease
Genes that were consistently up-regulated in KD patients compared with healthy controls, and that showed over 2.0 or 3.0-fold differences by the comparison between the two groups in the mean expression levels were selected. Microarray analysis for PBMNCs of acute-phase KD patients was performed usin...
ORGANISM(S): Homo sapiens 
2010-09-04 | E-GEOD-17975 | biostudies-arrayexpress
ProteomicsProximity biotinylation of A431 cells by EGFR-FabID
A431 cells were pretreated with DMSO or gefitinib and unstimulated or stimulated with EGF. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were puri...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD039450 | JPOST Repository
ProteomicsTMTpro-18plex analysis of HEK293T cells transfected with DCAF siRNAs
HEK293T cells transfected with control, CUL4, COP1, DCAF3, DCAF14, or RBBP7 siRNA were lysed in 150 µL guanidine buffer. After heating and sonication, the lysates were centrifuged at 20,000 × g for 15 min at 4°C. The supernatants were recovered, and proteins (150 µg each) were purified by methanol-c...
ORGANISM(S): Homo Sapiens (human) 
2025-11-14 | PXD057876 | JPOST Repository
ProteomicsProximity biotinylation of HEK293T cells stably expressing AirID-DCAFs
HEK293T cells stably expressing AGIA-AirID-DCAF, -SKP2, and -IκBα were cultured in a 10-cm dish in biological replicates (n = 2) and treated with 10 µM MG132 for 6 h in the presence of 10 µM biotin for 24 h. The cell pellet was then lysed in 300 µL of a guanidine buffer (6 M guanidine-HCl, 100 mM HE...
ORGANISM(S): Homo Sapiens (human) 
2025-11-14 | PXD057875 | JPOST Repository
ProteomicsDIA-based quantitative proteomics of HEK293T cells treated with MLN4294
HEK293T cells were cultured in 6-well plates and treated with DMSO or 2 µM MLN4924. After 6 h (MLN4924) or 24 h (DMSO or MLN4924), the cells (n=3) were collected using 1 mL of PBS by pipetting. All samples were centrifuged at 500 × g for 3 min at 4°C, and cell pellets were lysed in 160 µL of guanidi...
ORGANISM(S): Homo Sapiens (human) 
2026-03-05 | PXD064494 | JPOST Repository
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