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TranscriptomicsExpression data from wild-type and Rybp-deficient ES cells
We used microarrays to investigate a global change in gene expression by conditional depletion of Rybp in mouse ES cells. Total RNAs were extracted from wild-type and Rybp-deficient ES cells, and were subjected to microarray analysis using Affymetrix GeneChip Mouse Genome 430A 2.0 arrays
ORGANISM(S): Mus musculus 
2012-04-03 | E-GEOD-32287 | biostudies-arrayexpress
TranscriptomicsGene expression profile in Yaf2 KD and/or RYBP KO ES cells.
Gene expression change by Yaf2 KD in wild type or RYBP KO ES cells. Total RNAs were extracted from the respective ES cells, and were subjected to microarray analysis using Affymetrix GeneChip Mouse Genome 430A 2.0 arrays
ORGANISM(S): Mus musculus 
2012-04-03 | E-GEOD-32288 | biostudies-arrayexpress
TranscriptomicsRybp ChIP-chip in mouse ES cells
Rybp binding in wild-type, Eed-KO, and Dnmt1-KO ES cells ChIP on chip analysis was carried out using the Mouse Promoter ChIP-on-chip Microarray Set (G4490A, Agilent-014716 and Agilent-014717, Palo Alto, Calif., USA). ESCs were subjected to ChIP assay using a Rybp antibody (Santa Cruz; H-115). Purifi...
ORGANISM(S): Mus musculus 
2012-04-03 | E-GEOD-32289 | biostudies-arrayexpress
TranscriptomicsExpression regulation by RYBP in mouse embryonic stem cells
Polycomb repressive complexes (PRCs) are important chromatin regulators of ES cell function. RYBP binds Polycomb H2A monoubiquitin ligases Ring1A and Ring1B, and has been suggested to participate in localizing Polycomb complexes to their targets. Moreover, constitutive inactivation of RYBP precludes...
ORGANISM(S): Mus musculus 
2012-04-03 | E-GEOD-32294 | biostudies-arrayexpress
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