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TranscriptomicsA Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT
Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcript...
ORGANISM(S): Saccharomyces cerevisiae 
2016-08-10 | E-GEOD-80831 | biostudies-arrayexpress
TranscriptomicsFACT and Spt6 Prevent Cryptic Transcription by Impeding H2A.Z Loading in Gene Bodies
H2A.Z is a highly conserved histone variant involved in several key nuclear processes. It is incorporated into promoters by SWR-C-related chromatin remodeling complexes, but whether it is also actively excluded from non-promoter regions is not clear. Here, we provide genomic and biochemical evidence...
ORGANISM(S): Saccharomyces cerevisiae 
2015-05-12 | E-GEOD-62880 | biostudies-arrayexpress
ProteomicsAdaptive epigenetic changes in histone variants and modifications rescue the fitness of Arabidopsis plants with dysfunctional histone chaperones
The aim was to characterize histone variants and PTMs in mutant plants with histone chaperone dysfunction using mass spectrometry-based approach. We found that the maintenance of genome stability via chromatin remodelling depends on a two-tiered process. The first level relies on a highly flexible m...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-08-10 | PXD046034 | Pride
Arabidopsis Immunochemistry. Post-translational modifications Mass spectrometry Histone chaperone complex Chromatin remodeling Histone variants 
GenomicsCoordination of histone chaperones for parental histone segregation and epigenetic inheritance
Coordination of histone chaperones for parental histone segregation and epigenetic inheritance
PRJNA996579 | ENA
GenomicsNRP histone chaperones promote the removal of histone variant H2A.Z
NRP histone chaperones promote the removal of histone variant H2A.Z
PRJNA525997 | ENA
GenomicsEssential histone chaperones collaborate to regulate transcription and chromatin integrity
Essential histone chaperones collaborate to regulate transcription and chromatin integrity
PRJNA674587 | ENA
TranscriptomicsA compendium of nucleosome and transcript profiles reveals determinants of chromatin architecture and transcription [transcript profiling]
Nucleosomes in all eukaryotes examined to date adopt a characteristic architecture within genes and play fundamental roles in regulating transcription, yet the identity and precise roles of many of the trans-acting factors responsible for the establishment and maintenance of this organization remain...
ORGANISM(S): Saccharomyces cerevisiae 
2013-05-22 | E-GEOD-44876 | biostudies-arrayexpress
UnknownStructure-function relationship of H2A-H2B specific plant histone chaperones.
Not available
S-EPMC6985425 | biostudies-literature
GenomicsChIP-seq of H2A.Z and its histone chaperones during the cell cycle
The aim of this study was to uncover cell cycle dependent chromatin binding of H2A.Z and its histone chaperones. U2OS cells were stably transfected with Twin-Strep-tagged H2A.Z, ANP32e, or YL1 constructs, and WT cells were used as a negative/background control. U2OS cells were synchronised in G1 or ...
ORGANISM(S): Homo sapiens 
2023-06-29 | E-MTAB-11617 | biostudies-arrayexpress
TranscriptomicsA compendium of nucleosome and transcript profiles reveals determinants of chromatin architecture and transcription [nucleosome profiling]
Nucleosomes in all eukaryotes examined to date adopt a characteristic architecture within genes and play fundamental roles in regulating transcription, yet the identity and precise roles of many of the trans-acting factors responsible for the establishment and maintenance of this organization remain...
ORGANISM(S): Saccharomyces cerevisiae 
2013-05-22 | E-GEOD-44877 | biostudies-arrayexpress
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