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ProteomicsStress granules proteome composition
Analysis of stress granules in HeLa cells, after exposure to different stimuli. Granules were collected by a newly devised enrichment method, described in manuscript. Proteomics sample preparation of granules was carried out using the Single-pot, solid phase-enhanced sample preparation, SP3 method (...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2022-11-02 | MSV000090634 | MassIVE
GenomicsChIP-Seq of NSL3 and MBD-R2 in Drosophila melanogaster S2 cells to investigate the function of Non-Specific Lethal (NSL) complex
The major H4K16 acetylase MOF has been found in two complexes, the Male-Specific Lethal (MSL) and the Non-Specific Lethal (NSL) complex. The latter one consists of at least 7 members: NSL1, NSL2, NSL3, MBD-R2, MCRS2, WDS, and MOF. To investigate it's function, we performed genome-wide profiling of N...
ORGANISM(S): Drosophila melanogaster 
2012-06-19 | E-MTAB-1085 | biostudies-arrayexpress
GenomicsChIP-seq profiling of RNA Polymerase II in Drosophila melanogaster S2 cells depleted of the non-specific lethal (NSL) complex
We show that the non-specific lethal (NSL) complex in Drosophila binds to housekeeping genes. Individual ChIP-qPCR experiments of NSL target genes indicated a role of the NSL complex in RNA Polymerase II (Pol II) recruitment to promoters. Consequently, we obtained ChIP-Seq profiles of the Pol-II-sub...
ORGANISM(S): Drosophila melanogaster 
2012-06-19 | E-MTAB-1084 | biostudies-arrayexpress
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