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TranscriptomicsReal-time quantitative PCR analysis of mouse immature CD4SP thymocytes
RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted immature CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was pe...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49270 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of mouse mature CD4SP thymocytes
RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted mature CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was perf...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49271 | biostudies-arrayexpress
TranscriptomicsReal-time quantitative PCR analysis of mouse total CD4SP thymocytes
RNA was isolated with the RNeasy kit (Qiagen) from electronically sorted total CD4SP thymocytes. RNA was reverse transcribed into cDNA by oligo(dT) priming with the QuantiTect Reverse transcription kit (Qiagen). The expression of 84 key genes involved in apoptosis, or programmed cell death was perfo...
ORGANISM(S): Mus musculus 
2013-07-27 | E-GEOD-49264 | biostudies-arrayexpress
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