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ProteomicsImmunoprecipitation followed by PRM to detect endogenous interaction between VGLL3 and EWSR1
For immunoprecipitation to detect the endogenous interaction between VGLL3 and EWSR1, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhib...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039092 | JPOST Repository
ProteomicsImmunnoprecipitation followed by PRM to detect endogenous interaction between VGLL3 and DDX5
For immunoprecipitation to detect the endogenous interaction between VGLL3 and DDX5, cardiac myofibroblasts were fixed with 0.1% PFA and lysed in IP buffer [20 mM HEPES­NaOH (pH 7.5)/1 mM EGTA/1 mM MgCl2/150 mM NaCl /5% glycerol/1% NP­40] containing protease inhibitors (1:100) and phosphatase inhibi...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039093 | JPOST Repository
ProteomicsNakaya_VGLL3-FLAG_IP-MS_QEplus
Cardiac myofibroblasts retrovirally transduced with FLAG-VGLL3 or control vector were fixed with 0.1% PFA and lysed in a HEPES-RIPA buffer (20 mM HEPES-NaOH [pH 7.5]/1 mM EGTA/1 mM MgCl2/150 mM NaCl/0.25% sodium deoxycholate/0.05% SDS/1% NP-40) containing protease inhibitors (1:100), phosphatase inh...
ORGANISM(S): Mus Musculus (mouse) 
2022-12-24 | PXD039091 | JPOST Repository
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