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MetabolomicsLipidCreator workbench to probe the lipidomic landscape - Targeted lipidomics analysis of Human Plasma samples and comparison with NIST SRM 1950 standard

Here we present LipidCreator, a software that fully supports targeted lipidomics assay development. LipidCreator offers a comprehensive framework to compute MS/MS fragment masses for over 60 lipid classes. LipidCreator provides all functionalities needed to define fragments, manage stable isotope...

2020-04-15 | MTBLS1375 | MetaboLights
GenomicsMediator Complex Regulates Alternative mRNA Processing via the Med23 Subunit
Mediator complex is an integrative hub for transcriptional regulation. Here we show that Mediator regulates alternative mRNA processing via its Med23 subunit. Combining tandem affinity purification and mass spectrometry, we identified a number of mRNA processing factors that bind to a soluble recomb...
ORGANISM(S): Homo sapiens 
2012-02-24 | E-GEOD-33887 | biostudies-arrayexpress
TranscriptomicsBRG1 recruitment by transcription factors MITF and SOX10 defines a specific configuration of regulatory elements in the melanocyte lineage (RNA-seq)
Microphthalmia-associated transcription factor (MITF) is the master regulator of the melanocyte lineage. By tandem affinity purification and mass spectrometry, we present a comprehensive characterisation of the MITF interactome comprising multiple novel cofactors involved in transcription, DNA repli...
ORGANISM(S): Homo sapiens 
2015-04-02 | E-GEOD-61966 | biostudies-arrayexpress
GenomicsBRG1 recruitment by transcription factors MITF and SOX10 defines a specific configuration of regulatory elements in the melanocyte lineage (ChIP-seq)
Microphthalmia-associated transcription factor (MITF) is the master regulator of the melanocyte lineage. By tandem affinity purification and mass spectrometry, we present a comprehensive characterisation of the MITF interactome comprising multiple novel cofactors involved in transcription, DNA repli...
ORGANISM(S): Homo sapiens 
2015-04-02 | E-GEOD-61965 | biostudies-arrayexpress
ProteomicsA novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics
Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has been successfully employed to map signaling networks in several large-scale studies. However its application has so far been restricted to the small number of Flp/FRT-recombination competent cell lines....
ORGANISM(S): Homo sapiens (Human) Mus musculus (Mouse) 
2016-01-05 | PXD002855 | Pride
Tandem affinity purification-mass spectrometry Protein-protein interaction Streptavidin-hemagglutinin-tag 
ProteomicsiMixPro: intelligent Mixing of Proteomes for elimination of false positives in affinity purification-mass spectrometry
Protein complexes are essential in most organizational and functional aspects of the cell. Different strategies currently exist to analyze such protein complexes by mass spectrometry, including affinity purification (AP-MS) and proximal labeling based strategies. However, the high sensitivity of cur...
ORGANISM(S): Homo sapiens (Human) 
2016-09-27 | PXD004246 | Pride
Human overexpression cell lines ap-ms silac 
GenomicsReciprocal interactions of human C10orf12 and C17orf96 with PRC2 revealed by BioTAP-XL crosslinking and affinity purification
Short-read sequencing of DNA fractions of the BioTAP-XL pull-downs on human EZH2 and interacting proteins. ChIP-seq-like pulldown/input samples
ORGANISM(S): Homo sapiens 
2014-02-01 | E-GEOD-53495 | biostudies-arrayexpress
ProteomicsIsolation of aphid-Potato leafroll virus (PLRV) proteins complexes from viruliferous insects using affinity purification-mass spectrometry
The control of insect borne disease is recognized as one of the major agricultural, animal and human health challenges of today. Viruses in the family Luteoviridae are phloem-limited, plant viruses that are vectored by aphids in a circulative manner. They are responsible for a wide-range of economic...
ORGANISM(S): Potato leafroll virus Myzus persicae 
2021-05-27 | PXD022167 | Pride
Aphid Phloem-limited plant virus Ap-ms. Potato pathogen Potato leafroll virus Plrv Luteoviridae Polerovirus Circulative transmission Myzus persicae 
ProteomicsMapping Myc Interactome by Micro-Affinity Purification Coupling Mass Spectrometry
Affinity purification-mass spectrometry (AP-MS) is a powerful approach for protein-protein interaction (PPI) analysis. Here, we report the development of a micro-scale immobilized metal affinity chromatography (m-IMAC) platform for rapid and reproducible purification of protein complexes prior to ma...
ORGANISM(S): Homo sapiens (Human) 
2026-06-09 | PXD073401 | Pride
Transcriptional regulation mechanisms Proteomics Affinity purification-mass spectrometry C-myc 
ProteomicsIdentification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry
Glycosaminoglycans (GAGs) are essential functional components of the extracellular matrix (ECM). Artificial GAGs like sulfated hyaluronan (sHA) exhibit pro-osteogenic properties and boost healing processes and hence are of high interest for supporting wound healing. Although sulfated GAGs (sGAGs) a...
ORGANISM(S): Homo sapiens (Human) 
2021-11-03 | PXD023362 | Pride
Hbmsc Pull-down approach Saos2/osteoblast cell line Biotin-gag Sulfated glycosaminoglycan derivatives Lc-ms/ms 
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