Human hek-293t - OmicsDI

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HEK-293T cell (68)
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Disease

Disease free (8)
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Technology Type

Mass Spectrometry (198)
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Affinity purification coupled with mass spectrometry proteomics (72)
Bottom-up proteomics (42)
Data-dependent acquisition (27)
Gel-based experiment (14)
Mass spectrometry (6)
Chemical cross-linking coupled with mass spectrometry proteomics (5)
Data-independent acquisition (5)
SWATH MS (5)
SRM/MRM (3)
Top-down proteomics (3)
Mass spectrometry assay (2)
Affinity proteomics (2)
All-ion fragmentation (1)

Instrument Platform

Illumina NovaSeq 6000 (40)
Illumina HiSeq 2500 (21)
NextSeq 500 (19)
Illumina HiSeq 4000 (16)
Illumina MiSeq (14)
Illumina HiSeq 2000 (13)
NextSeq 550 (9)
Illumina HiSeq 1500 (8)
Illumina NovaSeq X (4)
PromethION (2)
Illumina Genome Analyzer II (2)
NextSeq 2000 (2)
KAPA EvoPrep Kit (1)
Bio-Rad Mini-PROTEAN electrophoresis system and wet transfer cassettes, (1)
Bio-Link Crosslinker BLX-E254 (1)
Sony MA-900 cell sorter (1)
BD FACSAria Fusion (1)
DNBSEQ Devices (1)
TriFast (VWR, 30-2010), RNeasy Mini Kit (Qiagen, 74104), DNAse 1 (NEB, M0303); Agilent 2100 Bioanalyzer (1)
HEK-293T cells, Media, PEI, Illumination device (1)
MGISEQ-2000RS (1)
Element AVITI (1)
Unknown (1)
E.Z.N.A. Total RNA Kit I (Omega Bio-tek) (1)
Illumina Genome Analyzier Iix (1)
MoFlo Legacy cell sorter (Beckman Coulter) (1)
PC (1)
BD FACSARIA III (1)
Fluidigm C1 (1)
Affymetrix Primeview Human Gene Expression Array (1)

Publication Date

2015 (122)
2014 (111)
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2013 (93)
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2012 (82)
2025 (75)
2023 (71)
2016 (68)
2020 (62)
2011 (56)
2017 (54)
2019 (53)
2018 (52)
2010 (46)
2026 (40)
2008 (23)
2009 (18)
2007 (11)
2006 (4)
2005 (1)

CHEBI ID

CHEBI:16750 (3)
CHEBI:17596 (3)
CHEBI:20129 (2)
CHEBI:17713 (2)
CHEBI:17200 (2)
CHEBI:17013 (2)
CHEBI:15713 (2)
CHEBI:16264 (2)
CHEBI:16695 (2)
CHEBI:20607 (2)
CHEBI:17802 (2)
CHEBI:17562 (2)
CHEBI:17361 (2)
CHEBI:16335 (2)
CHEBI:16704 (2)
CHEBI:16027 (2)
CHEBI:17345 (2)
CHEBI:16020 (2)
CHEBI:21167 (1)
CHEBI:60784 (1)
CHEBI:140004 (1)
CHEBI:133090 (1)
CHEBI:57345 (1)
CHEBI:16155 (1)
CHEBI:28072 (1)
CHEBI:62501 (1)
CHEBI:35850 (1)
CHEBI:15375 (1)
CHEBI:47191 (1)
CHEBI:23858 (1)

Metabolite Name

Guanosine (3)
3-methylcytidine (2)
5-methylcytidine (2)
Uridine (2)
Pseudouridine (2)
Inosine (2)
Cytidine (2)
Adenosine (2)
GDP-Hexose (1)
UDPAG (1)
Thymidylic acid (1)
Guanylate (1)
5'-adenylic acid (1)
Uridine-diphosphate (1)
Cytidylic acid (1)
N6-,O2'dimethyladenosine (1)
N4-Methylcytidine (1)
UDP-N-acetylgalactosamine (1)
Tricosenoate (23:1) (1)
Sphingomyelin (d18:2/14:0, d18:1/14:1)* (1)
Sphingomyelin (d18:1/15:0, d16:1/17:0)* (1)
S-nitrosoglutathione (GSNO) (1)
N-acetylgalactosamine (1)
Mesaconate (methylfumarate) (1)
Isovaleryl CoA (1)
Isobar: fructose 1,6-diphosphate, glucose 1,6-diphosphate, myo-inositol 1,4 or 1,3-diphosphate (1)
Diadenosine triphosphate (1)
Behenate (22:0)* (1)
Alpha-CEHC sulfate (1)
7-hydroxycholesterol (alpha or beta) (1)

First Public Date

2024 (25)
2025 (11)
2021 (10)
2017 (10)
2026 (8)
2023 (8)
2019 (8)
2015 (8)
2020 (6)
2022 (5)
2014 (5)
2016 (3)
2018 (2)
2013 (1)

Modification

15.994915 (6)
57.021464 (6)
71.037114 (3)
2x(15)N labeled L-lysine (1)
4x(15)N labeled L-arginine (1)
4x(2)H labeled dimethylated residue (1)
6.020129 (1)
79.966331 (1)

Study type

Transcription profiling by array (327)
RNA-seq of coding RNA (167)
ChIP-seq (84)
Other (42)
RNA-seq of non coding RNA (23)
Cell line - High-throughput sequencing (17)
RNA-seq of total RNA (12)
ChIP-chip by tiling array (11)
CLIP-seq (11)
Methylation profiling by high throughput sequencing (10)
Methylation profiling by array (7)
CUT&RUN (6)
RNA-seq of coding RNA from single cells (6)
ATAC-seq (5)
RNAi profiling by array (4)
RIP-seq (4)
DNA-seq (4)
Proteomic profiling by array (4)
ChIP-chip by array (3)
Unknown experiment type (2)
Comparative genomic hybridization by array (2)
Ribo-seq (2)
Genotyping by high throughput sequencing (2)
Transcription profiling by high throughput sequencing (1)
GRO-seq (1)
Transcription profiling by SAGE (1)
Baseline (1)
RIP-chip by array (1)
High-throughput sequencing (1)
Animal - High-throughput sequencing (1)

Release Date

2015 (121)
2014 (117)
2013 (96)
2012 (86)
2016 (71)
2021 (62)
2011 (62)
2022 (58)
2010 (51)
2017 (44)
2020 (42)
2018 (39)
2025 (38)
2024 (33)
2023 (30)
2019 (25)
2009 (25)
2008 (25)
2026 (18)
2007 (13)
2006 (7)
2005 (3)
2004 (3)
2003 (1)

Lab affiliation

Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA (3)
Department of Biology – Cellular Proteostasis and Mass Spectrometry, University of Konstanz (3)
Department of pathophysiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China (3)
(1) German Center for Neurodegenerative Diseases (DZNE), 72076 Tübingen, Germany (2) University of Tübingen, Center for Ophthalmology, Institute for Ophthalmic Research, 72074 Tübingen, Germany (2)
Cornell University (2)
Dana Farber Cancer Institute (2)
Department of Gastroenterology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, China. (2)
Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA (2)
Dept. of Molecular Biosciences, University of Texas at Austin, Texas 78712 USA (2)
Edinburgh Cancer Research, CRUK Scotland Centre, Institute of Genetics and Cancer, University of Edinburgh, Crewe Road South, Edinburgh EH4 2XU, UK (2)
Experimental Radiation Oncology, MD Anderson Cancer Center, Houston, TX, USA (2)
Faculty of Chemistry and Pharmacy, Biochemistry II, Theodor-Boveri-Institute, University of Wuerzburg, Germany (2)
IMol Polish Academy of Sciences, Warsaw, Poland (2)
Molecular Biosciences and the Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, TX USA (2)
Protein Analysis Facility, Faculty of Biology and Medicine, University of Lausanne, Switzerland (2)
ReMedy International Research Agenda Unit, IMol Polish Academy of Sciences, Warsaw, Poland (2)
Shanghai Jiao Tong University (2)
Sir William Dunn School of Pathology, University of Oxford (2)
Whitehead Institute for Biomedical Research, Cambridge, MA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA (2)
Yale University (2)
1) Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York, 10065, USA. 2) Pharmacology Program of the Weill Cornell Graduate School of Medical Sciences, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA. 3) Tri-Institutional PhD Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, New York 10065, USA. (1)
1) German Center for Neurodegenerative Diseases (DZNE), 72076 Tübingen, Germany (2) University of Tübingen, Center for Ophthalmology, Institute for Ophthalmic Research, 72074 Tübingen, Germany (1)
1Department of Biochemistry and Biophysics, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States (1)
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093 (1)
1Gavin Herbert Eye Institute –Brunson Center for Translational Vision Research, Department of Ophthalmology and Visual Sciences, University of California Irvine, Irvine, CA 92697, USA 2Department of Physiology and Biophysics, University of California Irvine, Irvine, CA 92697, USA 3Department of Chemistry, University of California Irvine, Irvine, CA 92697, USA 4Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA 92697, USA (1)
Assistant professor, School of Biological Sciences, Seoul National University (1)
Associate Professor Developmental and Regenerative Biology School of Biomedical Sciences Faculty of Medicine The Chinese University of Hong Kong (1)
Associate Professor of Chemistry and Chemical Biology Department of Chemistry and Chemical Biology Harvard University, USA (1)
BCMP, Harvard Medical School, Boston, USA Cancer Biology, Dana-Farber Cancer Institute, Boston, USA (1)
Baxter Laboratory for Stem Cell Biology Department of Microbiology & Immunology Stanford University School of Medicine (1)

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Role of BACH1 in HEK 293T cells

This SuperSeries is composed of the following subset Series: GSE28050: Expression data from knockdown of BACH1 in HEK 293T cells GSE28051: Genome-wide map of BACH1 binding in HEK293T cells Refer to individual Series

ORGANISM(S): Homo sapiens

2011-05-12 | E-GEOD-28053 | biostudies-arrayexpress

Lipids in the escalated host-pathogen arms race

Lipids, fundamental constituents of cell membranes, cellular energy carriers, and signaling molecules, play a pivotal role in the interaction between pathogens and hosts. During microbial infections, lipids not only fuel the activation of the immune system but also, acting as signaling molec...

2025-07-05 | MTBLS12678 | MetaboLights

RNA-seq of human HEK-293T cells treated with a nutraceutical formula (solution-10), or vehicle-(saline solution)- control

HEK-293T cells were plated (1x106 cells) and treated with a nutraceutical formula (Solution-10) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment. After 24 hours, the cells were lysed, and their RNA was extracted for RNAseq.

ORGANISM(S): Homo sapiens

2026-08-14 | E-MTAB-17521 | biostudies-arrayexpress

Genome-wide map of BACH1 binding in HEK 293T cells

BTB and CNC homology 1 (BACH1) is a heme-binding transcription factor repressing the transcription from a subset of MAF recognition elements (MAREs) at low intracellular heme levels. Upon heme binding, BACH1 is released from the MAREs, resulting in increased expression of antioxidant response genes....

ORGANISM(S): Homo sapiens

2011-05-13 | E-GEOD-28051 | biostudies-arrayexpress

RNA-seq of human HEK-293T cells treated with a nutraceutical formula (solution-3), or vehicle-(saline solution)-treated controls

HEK-293T cells were plated (1x106 cells) and treated with a nutraceutical formula (solution-3) at 0.01x concentration or with vehicle (saline solution) as the negative control for the treatment. After 24 hours, the cells were lysed, and their RNA was extracted for RNAseq.

ORGANISM(S): Homo sapiens

2024-06-19 | E-MTAB-13918 | biostudies-arrayexpress

Gene expression data from HEK-293T cells transfected with miR-194 mimics

Nuclear factor κB (NF-κB) pathway plays an important role in hepatocellular carcinoma (HCC) progression. miR-194 was previously shown to reduce the induction of NF-κB activity upon addition of tumor necrosis factor α (TNFα). To clarify the molecular mechanism responsible for the effect of miR-194 on...

ORGANISM(S): Homo sapiens

2015-07-01 | E-GEOD-70434 | biostudies-arrayexpress

Expression data from knockdown of BACH1 in HEK 293T cells

ORGANISM(S): Homo sapiens

2011-05-13 | E-GEOD-28050 | biostudies-arrayexpress

HEK 293T cells RNA-seq data treated with 5-aza-2-deoxycytidine

DNA methylation can be abnormally regulated in human disease and associated with effects on gene transcription that appear to be causally related to pathogenesis. The potential to use pharmacological agents that reverse this dysregulation is therefore an attractive possibility. To test how 5-aza-2...

ORGANISM(S): Homo sapiens

2015-02-13 | E-GEOD-62591 | biostudies-arrayexpress

Alteration of gene expression in HEK 293T cells upon inhibition of PCAF by embelin

KAT2B (PCAF) belongs to GNAT family of lysine acetyltransferases (KAT), which specifically acetylates histone H3K9 residue and several nonhistone proteins. PCAF is also a transcription coactivator. Due to the lack of PCAF, KAT specific small molecule inhibitor, the exclusive role of the acetyltransf...

ORGANISM(S): Homo sapiens

2013-03-31 | E-GEOD-39828 | biostudies-arrayexpress

Shotgun analysis of proteins in NDUFA11 KO and WT HEK 293T cells.

The effect of the depletion of an ETC complex I protein component - NDUFA11 on the proteome in HEK 293T cells was studied. TMT-based relative quantification of protein levels were performed in NDUFA11 KO vs WT cells and in NDUFA11 KO vs WT cells that expressed an EGFP-MAPT (Tau protein) construct. I...

ORGANISM(S): Homo sapiens (Human)

2023-07-03 | PXD038397 | Pride

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