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MetabolomicsExamining the effects of cigarette smoke on mouse lens through a multi OMIC approach
Here, we report a multi OMIC (transcriptome, proteome, and metabolome) approach to investigate molecular changes in lens fiber cells (FC) of mice exposed to cigarette smoke (CS). Pregnant mice were placed in a whole-body smoke chamber and a few days later pups were born, which were exposed to CS for...
2024-03-24 | MTBLS207 | MetaboLights
MetabolomicsQuantitative metabolomic analysis of changes in the lens and aqueous humor under development of age-related nuclear cataract

INTRODUCTION: Metabolites are essential for the proper functioning of the eye lens, they either enter the lens from the aqueous humor (AH), or are synthesized in the lens epithelium. Antioxidants, osmolytes and UV filters are especially important for the lens protection, and thei...

2022-11-07 | MTBLS740 | MetaboLights
MetabolomicsThe role of FYCO1-dependent autophagy in lens fiber cell differentiation.

FYCO1 (FYVE and coiled-coil domain containing 1) is an adaptor protein, expressed ubiquitously and required for microtubule-dependent, plus-end-directed transport of macroautophagic/autophagic vesicles. We have previously shown that loss-of-function mutations in FYCO1 cause cataracts wit...

2024-03-24 | MTBLS206 | MetaboLights
TranscriptomicsTranscription profiling of human lens epithelial cells to examine glucocorticoid induced changes
Prolonged use of glucocorticoids can lead to the formation of a cataract, however the mechanism is not known. We recently reported the presence of the functional glucocorticoid receptor in immortalized cultured mammalian lens epithelial cells (Gupta & Wagner, Invest Ophthalmol Vis Sci 2003), but the...
ORGANISM(S): Homo sapiens 
2007-09-01 | E-GEOD-3040 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human lens epithelial cells and lens cortical fiber cells
Total RNA was isolated from three separate populations of human lens epithelial cells and three matching populations of lens cortical fiber cells. All samples were analyzed on separate microarrays.
ORGANISM(S): Homo sapiens 
2007-08-25 | E-GEOD-2256 | biostudies-arrayexpress
MetabolomicsNMR-Based Metabolomics of Non-Human Primate Ocular Tissues: A Dataset
Model animals are employed in experiments as substitutes for human tissues and fluids, particularly when accessing particular human samples (such as cerebrospinal fluid, brain, ocular tissues, etc.) poses significant challenges or is ethically constrained. Nonhuman primates are frequently regarded a...
2023-11-16 | MTBLS8943 | MetaboLights
ProteomicsAge-related cleavages of crystallins in human lens cortical fibre cells generate a plethora of low molecular weight peptides and cross-linked crystallin complexes
A large variety of low molecular weight (LMW) peptides, derived from the breakdown of crystallins, have been reported in middle to old age human lenses. The proliferation of these LMW peptides coincides with the earliest stages of cataract formation, suggesting that the protein cleavages involved ma...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
MSV000081536 | MassIVE
TranscriptomicsDevelopmental timecourse of mouse ocular lens and whole embryo control
Identification of genes involved in ocular birth defects remains a challenge. To facilitate the identification of genes associated with cataract, we developed iSyTE (integrated Systems Tool for Eye gene discovery; http://bioinformatics.udel.edu/Research/iSyTE). iSyTE contains microarray gene express...
ORGANISM(S): Mus musculus 
2012-02-16 | E-GEOD-32334 | biostudies-arrayexpress
ProteomicsBasement Membranes from Human Retinal Blood Vessels, Inner Limiting Membranes, and Lens Capsules
Three types of BMs from adult human eyes, the inner limiting membrane, the retinal vascular BMs and the lens capsule, were isolated for analysis by 1D-SDS-PAGE and LC-MS/MS.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-11-29 | MSV000081772 | MassIVE
MetabolomicsMetabolomics of the human aqueous humor
Introduction: The optical elements of the eye – cornea, lens, and vitreous humor – are avascular tissues, and their nutrition and waste removal are provided by aqueous humor (AH). The AH production occurs through the active secretion and the passive diffusion/ultrafiltration of blood plasma. The com...
2017-11-06 | MTBLS386 | MetaboLights
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