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Gene expression studies comparing IFNg+ Tregs versus IFNg- Tregs from human peripheral blood Ex vivo sorted Tregs (CD25highCD127neg) were stimulated for 4 hours and IFNg-secreting cells were detected by a IFNg-capture kit. The samples were resorted based on IFNg expression.
ORGANISM(S): Homo sapiens 
Microarray used to detail the global gene transcription underlying sorted IFNg+ and IFNg- Tregs (CD4+CD25+CD127lo) and Tconv (CD4+CD25-CD127+) for fresh (unexpanded) and 14 day expanded cells from human blood. Treg and Tconv were FACS isolated from five healthy subjects (median age of 26, range 22-3...
ORGANISM(S): Homo sapiens 
CD70TG mice are a model for sterile chronic immune activation and develop Anemia of Inflammation, which is dependent on the production of Ifng by effector CD4 and CD8 T cells. We used microarrays to identify Ifng-dependent differentially expressed genes that could account for the erythropoietic defe...
ORGANISM(S): Mus musculus 
This experiment was designed to identify IFNg-regulated, IRF1-dependent genes during infection with the intracellular pathogen Shigella flexneri. WT and Irf1-/- MEFs were stimulated for 18 hours with IFNg or left unstimulated; all of the cells were subsequently infected for 6 hours with S. flexneri...
ORGANISM(S): Mus musculus 
IFNg treatment of monocytes
In order to fully characterize emodin's effects on macrophage activation, peritoneal macrophages were stimulated with LPS+IFNg with or without emodin and gene expression was analyzed using a whole genome microarray. Emodin significantly attenuated the IFNg/LPS induced changes in a large percentage o...
ORGANISM(S): Mus musculus 
Human neutrophils stimulated with IFNg.
NGS5522 - hMDMS response to IFNg and TNFa
Cell specific response to IFNg
Mapping of chromatin anchors within the human Major Histocompatability Complex (MHC) region from B-lymphoblastoid cells and IFNg+/IFNg- treated fibroblasts.
ORGANISM(S): Homo sapiens 
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