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MetabolomicsMetabolomics profiling (GC-MS and LC-MS) for time-course differentiation of pre-B cells-like (B3 cell line) under the controlled induction of the transcription factor Ikaros (STATegra Project)
The main goal of the project is to develop a new generation of bioinformatics resources for the integrative analysis of multiple types of 'omics data. These resources include both novel statistical methodologies as well as user-friendly software implementations. STATegra methods address many aspects...
2016-12-15 | MTBLS283 | MetaboLights
TranscriptomicsGene expression in mouse pre-B cells transduced with Ikaros.
Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in pre-B cells we performed gene expression studies at enhanced temporal resolution. We used retroviral gene transfer to express Ikaros proteins in the pre-B cell line B3. Total RNA f...
ORGANISM(S): Mus musculus 
2013-01-15 | E-GEOD-38110 | biostudies-arrayexpress
TranscriptomicsGene expression in mouse primary pre-B cells transduced with Ikaros
Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in primary pre-B cells we performed gene expression microarrays. We used retroviral gene transfer to express Ikaros proteins. Total RNA from 3 biological replicates of primary pre-B c...
ORGANISM(S): Mus musculus 
2013-01-15 | E-GEOD-42551 | biostudies-arrayexpress
TranscriptomicsConcerted action of Helios and Ikaros balances the strength of B cell receptor signaling and controls the expression of inositol 5-phosphatase SHIP
Inositol 5-phosphatase SHIP is differentially expressed in Ikaros and Helios deficient cells. In the absense of Ikaros SHIP is upregulated where as in the absense of Helios it is downregulated. Ikaros binds to the promoter of the SHIP gene. Article provides insight into the mechanisms of action Ikar...
ORGANISM(S): Gallus gallus 
2010-07-19 | E-GEOD-20946 | biostudies-arrayexpress
TranscriptomicsStage-specific control of early B cell development by the transcription factor Ikaros
Ikaros is an essential regulator of lymphopoiesis. Here, we studied the B-cell-specific function of Ikaros by conditional Ikzf1 inactivation in pro-B cells. B-cell development was arrested at an aberrant ‘pro-B’ cell stage characterized by increased cell adhesion and loss of pre-B cell receptor sign...
ORGANISM(S): Mus musculus 
2014-02-09 | E-GEOD-53595 | biostudies-arrayexpress
GenomicsGenome-wide identification of Ikaros binding sites in primary pre-B cells
Ikaros DNA binding proteins are important regulators of haematopoiesis and genetic deletion of Ikaros results in severe developmental disturbances, including delayed thymocyte differentiation and an early and complete block in B cell development. Although Ikaros ChIP-seq data are available for mouse...
ORGANISM(S): Mus musculus 
2013-01-15 | E-GEOD-42462 | biostudies-arrayexpress
TranscriptomicsExpression profiling of mouse T-ALL (Vav-tTA;TRE-GFP-shIkaros primary leukemia ALL101) cells following Ikaros restoration [RNA-Seq]
To examine Ikaros tumor suppressor mechanisms, we have utilized inducible RNAi to dynamically restore endogenous Ikaros expression in T-ALL driven by its knockdown. This causes rapid transcriptional repression of Notch1 and associated targets including Myc, even in leukemias harboring spontaneous ac...
ORGANISM(S): Mus musculus 
2015-02-02 | E-GEOD-54447 | biostudies-arrayexpress
GenomicsMechanisms of Ikaros Tumor Suppression with HDAC1 through distinct histone markers in Pre-B ALL Leukemia
Ikaros functions as a master regulator of lymphocyte differentiation and a tumor suppressor. Ikaros binds DNA and regulates gene expression by chromatin remodeling. Mechanisms of Ikaros-mediated tumor suppression are unknown. Here we examined genome-wide occupancy of Ikaros and histone deacetylase 1...
ORGANISM(S): Homo sapiens 
2015-11-03 | E-GEOD-44217 | biostudies-arrayexpress
GenomicsGenome-wide identification of Ikaros binding sites in pre-B cells
Ikaros DNA binding proteins are important regulators of haematopoiesis and genetic deletion of Ikaros results in severe developmental disturbances, including delayed thymocyte differentiation and an early and complete block in B cell development. Although Ikaros ChIP-seq data are available for mouse...
ORGANISM(S): Mus musculus 
2013-01-15 | E-GEOD-38169 | biostudies-arrayexpress
TranscriptomicsWT and Ikaros-deficient follicular B cells stimulated with anti-IgM
We have observed that follicular B cells from mice with a hypomorphic mutation (IkL/L) in the Ikzf1 gene (which encodes the Ikaros transcription factor) exhibit an increased proliferative response to anti-IgM stimulation (Kirstetter et al, Eur J Immunol, 32:720-30, 2002). We asked if Ikaros controls...
ORGANISM(S): Mus musculus 
2016-01-26 | E-GEOD-75712 | biostudies-arrayexpress
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