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GenomicsGenotyping of E14 mouse embryonic stem cells by sequencing
More than 2x10E9 sequences made on Illumina platform derived from the genome of E14 embryonic stem cells cultured in our laboratory were used to build a database of about 2.7x10E6 single nucleotide variant. The database was validated using other two sequencing datasets from other laboratory and high...
ORGANISM(S): Mus musculus 
2014-07-10 | E-GEOD-53149 | biostudies-arrayexpress
TranscriptomicsRole of miRNAs in acute kidney injury recovery induced by mesenchymal stromal cells and derived extracellular vesicles.
Phenotypic changes induced by extracellular vesicles (EVs) have been implicated in the recovery of acute kidney injury (AKI) induced by mesenchymal stromal cells (MSCs). miRNAs are potential candidates for cell reprogramming towards a pro-regenerative phenotype. The aim of the present study was to e...
ORGANISM(S): Mus musculus 
2015-05-07 | E-GEOD-59958 | biostudies-arrayexpress
GenomicsSingle base resolution analysis of 5-formyl and 5-carboxyl cytosine reveals the actual promoter DNA methylation dynamics in embryonic stem cells [ChIP-Seq]
DNA methylation is catalysed by DNA methyltransferases (DNMTs) and is necessary for a correct embryonic development. On the other hand, the DNA demethylation is mediated by the Ten Eleven Translocation (Tet) proteins through oxidation of 5-methyl cytosine (5mC) to 5-hydroxyl (5hmC), 5-formyl (5fC) a...
ORGANISM(S): Mus musculus 
2015-02-10 | E-GEOD-55657 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of mouse RNA secondary structures reveals key features of the mammalian transcriptome
By using a novel high-throughput method, named chemical inference of RNA structures (CIRS-seq), that uses dimethyl sulfate (DMS), and N-cyclohexyl-N'-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate (CMCT) to modify RNA residues in single-stranded conformation within native RNA secondary str...
ORGANISM(S): Mus musculus 
2014-10-01 | E-GEOD-54106 | biostudies-arrayexpress
GenomicsSingle base resolution analysis of 5-formyl and 5-carboxyl cytosine reveals the actual promoter DNA methylation dynamics in embryonic stem cells [MAB-Seq]
DNA methylation is catalysed by DNA methyltransferases (DNMTs) and is necessary for a correct embryonic development. On the other hand, the DNA demethylation is mediated by the Ten Eleven Translocation (Tet) proteins through oxidation of 5-methyl cytosine (5mC) to 5-hydroxyl (5hmC), 5-formyl (5fC) a...
ORGANISM(S): Mus musculus 
2015-02-10 | E-GEOD-55658 | biostudies-arrayexpress
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