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We determined the cryo-EM structure of the type III secretion injectisome needle complex. We used LC-MS/MS to idensitfy the constituent proteins showing the predominant presence of the major inner- and outer basal body proteins (PrgH+PrgK and InvG respectively), export apparatus protein SpaP and Spa...
ORGANISM(S): Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
2018-10-16 | PXD010239 | Pride
We determined the cryo-EM structure of a secretion incompetent PrgH130-392 type III secretion injectisome basal body mutant at 6.3 Å. The map shows no density attributable to the inner rod or needle and the periplasmic gate in a closed conformation. We used LC-MS/MS to idensitfy the constituent prot...
ORGANISM(S): Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
2016-12-19 | PXD005133 | Pride
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