Single-cell metabolomics reveals cell heterogeneity and elucidates intracellular molecular mechanisms. However, general concentration measurement of metabolites can only provide a static delineation of metabolomics, lacking the metabolic activity information of biological pathways. Herein, we dev...

Microbiota-released extracellular vesicles (MEVs) have emerged as a key player in intercellular signaling. However, their involvement in the gut-brain axis has been poorly investigated. In this study, we aimed to investigate the cargo capacity of MEVs for bioactive metabolites and their interacti...

Maintaining the integrity of the mucus layer is essential for host health. Studies have implicated that reduced or abnormal mucus production may lead to intestinal inflammation. When dietary nutrients are abundant in the gut, mucin degradation by microbes decreases. However, when nutrient availab...

Depression is an individual risk factor for poor prognosis in patients with heart failure (HF). Recent studies show that gut microbiota and metabolites have critical role in comorbid HF and depressive symptoms. We recruited 95 subjects including 35 HF patients with depressive symptoms (HF-DS), 36...

This study presents a time-resolved untargeted LC–MS/MS metabolomics dataset of the interaction between melon (Cucumis melo) and the obligate biotrophic fungus Podosphaera xanthii, the causal agent of cucurbit powdery mildew. Mock-treated and inoculated plants were sampled every 12 hours from 12 ...

Disruption of organelle interactions due to metabolic stress is a crucial factor in the pathological processes of many degenerative diseases. Current treatments usually focus on individual organelles while ignoring the role of organelle interactions in cell damage caused by cascade reactions. The...

Genetic interactions are fundamental to the architecture of complex traits, yet the molecular mechanisms by which variant combinations influence cellular pathways remain poorly understood. Here, we answer the question of whether interactions between genetic variants can activate unique pathways a...

Poplar seedlings were cultivated in saline soil with low salt concentrations for 120 days, after which root tissues were harvested for untargeted metabolomic analysis. For each genotype, roots of three to four seedlings were pooled to form one biological replicate, with four biological replicates...