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TranscriptomicsNovel Observations from Next Generation RNA Sequencing of Highly Purified Human Adult and Fetal Islet Cell Subsets
Understanding distinct gene expression patterns of normal adult and developing fetal human pancreatic a and b cells is crucial for developing stem cell therapies, islet regeneration strategies, and therapies designed to increase b cell function in patients with diabetes (type 1 or 2). Toward that en...
ORGANISM(S): Homo sapiens 
2015-06-08 | E-GEOD-67543 | biostudies-arrayexpress
TranscriptomicsRNA-sequencing identifies dysregulation of the human pancreatic islet transcriptome by the saturated fatty acid palmitate
Pancreatic beta-cell dysfunction and death are central in the pathogenesis of type 2 diabetes. Saturated fatty acids cause beta-cell failure and contribute to diabetes development in genetically predisposed individuals. Here we used RNA-sequencing to map transcripts expressed in five palmitate-treat...
ORGANISM(S): Homo sapiens 
2014-01-09 | E-GEOD-53949 | biostudies-arrayexpress
GenomicsDynamic epigenetic regulation by menin during pancreatic islet tumor formation
loss of Men1 in mouse pancreatic islet cells alters the epigenetic landscape of a subset of target genes. H3K4me3 ChIP-seq from either mouse pancreatic islets or mouse pancreatic islet tumors harvested at different stages.
ORGANISM(S): Mus musculus 
2015-04-16 | E-GEOD-63020 | biostudies-arrayexpress
GenomicsHeterogeneous endocrine cell composition defines human islet functional phenotypes
Heterogeneous endocrine cell composition defines human islet functional phenotypes
PRJNA1513429 | ENA
GenomicsHeterogeneous endocrine cell composition defines human islet functional phenotypes
Heterogeneous endocrine cell composition defines human islet functional phenotypes
PRJNA1513430 | ENA
TranscriptomicsSingle-cell transcriptomics reveals unique features of human pancreatic islet cell subtypes
We report the single-cell RNA-seq based identification of 6 known human islet cell types (alpha cells, beta cells, delta cells, pp cells, acinar cells and duct cells) based on the expression of known marker genes. We further assess cell type specific gene expression and suggest novel marker genes fo...
ORGANISM(S): Homo sapiens 
2016-01-05 | E-GEOD-73727 | biostudies-arrayexpress
TranscriptomicsUse of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells
This study provides an assessment of the Fluidigm C1 platform for RNA sequencing of single mouse pancreatic islet cells. The system combines microfluidic technology and nanoliter-scale reactions. We sequenced 622 cells allowing identification of 341 islet cells with high-quality gene expression prof...
ORGANISM(S): Mus musculus 
2016-02-19 | E-GEOD-77980 | biostudies-arrayexpress
TranscriptomicsGlobal transcriptomic analysis of human pancreatic islets reveals novel genes influencing glucose metabolism [RNA-seq]
Here we harnessed the potential of RNA sequencing in 89 human pancreatic islet donors to identify genes and exons regulated in this relevant tissue for T2D. mRNA profiles of 89 human pancreatic islet donors having different levels of blood glucose (HbA1c) with and without T2D. The data was generated...
ORGANISM(S): Homo sapiens 
2014-07-17 | E-GEOD-50244 | biostudies-arrayexpress
GenomicsControlling human stem cell-derived islet composition using magnetic sorting
Controlling human stem cell-derived islet composition using magnetic sorting
PRJNA1168949 | ENA
TranscriptomicsRNA seq of pancreatic islets isolated from free fatty acid receptor 3 knockout (Ffar3 KO) and wildtype (Ffar3 WT) male mice
The short chain fatty acid (SCFA) receptor (free fatty acid receptor-3; FFAR3) is expressed in pancreatic beta cells; however, its role in insulin secretion is not clearly defined. Here, we examined the role of FFAR3 in insulin secretion. Using islets from global knockout FFAR3 (Ffar3-/-) mice, we e...
ORGANISM(S): Mus musculus 
2016-07-03 | E-GEOD-67991 | biostudies-arrayexpress
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