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MetabolomicsIsotope tracing-based metabolite identification for mass spectrometry metabolomics

Modern mass spectrometry-based metabolomics is a key technology for biomedicine, enabling discovery and quantification of a wide array of biomolecules critical for human physiology. Yet, only a fraction of human metabolites have been structurally determined, and the majority of features in typica...

2025-08-15 | MTBLS12861 | MetaboLights
MetabolomicsLC-MS-based isotope tracing data supporting cholesterol- and EA2-regulated fatty acid synthesis and tumor metabolic remodeling
This study contains LC-MS-based stable isotope tracing data investigating cholesterol- and EA2-regulated tumor metabolic remodeling. The dataset includes U-13C-glucose tracing in tumor cells and D2O tracing in animal models, supporting analysis of cholesterol-driven metabolic remodeling and the supp...
2026-10-03 | MTBLS14937 | MetaboLights
MetabolomicsA Comprehensive and Ultrasensitive Isotope Calibration Method for Soil Amino Compounds Using Orbitrap Mass Spectrometry
Bound amino compounds (amino acid and amino sugar polymers) comprise a significant proportion (~40%) of soil organic nitrogen and therefore represent an essential source of nitrogen for plant and microbial nutrition. The analysis of their content and isotope enrichment still represents a significant...
2025-06-16 | MTBLS12550 | MetaboLights
MetabolomicsMatrix Effect on Chemical Isotope Labeling and Its Implication in Metabolomic Sample Preparation for Quantitative Metabolomics
Matrix effect from various constituents in biological samples can reduce the accuracy of quantitative metabolomics. Differential chemical isotope labeling liquid chromatography mass spectrometry (CIL LC-MS) can overcome the matrix effect on MS detection based on measuring the intensity ratios of met...
2015-07-01 | MTBLS194 | MetaboLights
MetabolomicsStable Isotope-Assisted Metabolomics for Deciphering Xenobiotic Metabolism in Mammalian Cell Culture
Xenobiotics are ubiquitous in the environment and modified abundantly in the human body by phase I and II metabolism. Liquid chromatography coupled to high resolution mass spectrometry is a powerful tool to investigate these biotransformation products. Here, we present a workflow based on stable iso...
2020-04-14 | MTBLS1358 | MetaboLights
MetabolomicsSteady state isotope tracing in C. elegans (GC-MS analyses)

The metabolic network, composed of thousands of metabolic reactions, is essential for any organism. Despite its importance, a systems level understanding of how metabolic network is wired by reactions that carry flux in unperturbed condition and rewired by transcriptionally activating or repressi...

2024-12-03 | MTBLS11742 | MetaboLights
MetabolomicsGlobal Stable-isotope Tracing Metabolomics Reveals System-wide Metabolic Alternations in Aging Drosophila

System-wide metabolic homeostasis is crucial for maintaining physiological functions of living organisms. Stable-isotope tracing metabolomics allows to unravel metabolic activity quantitatively by measuring the isotopically labeled metabolites, but has been largely restricted by coverage. Yet, de...

2022-06-16 | MTBLS3322 | MetaboLights
MetabolomicsSteady state isotope tracing in C. elegans (LC-MS analyses)

The metabolic network, composed of thousands of metabolic reactions, is essential for any organism. Despite its importance, a systems level understanding of how metabolic network is wired by reactions that carry flux in unperturbed condition and rewired by transcriptionally activating or repressi...

2024-12-03 | MTBLS11741 | MetaboLights
MetabolomicsStable isotope labelling of Toxoplasma gondii under iron and copper restriction

Toxoplasma uses metal nutrients like iron and copper for vital functions in their energy metabolism. Here, we have used stable isotope labelling to track the incorporation of glucose and glutamine into metabolic pathways, primarily focusing on central carbon metabolism, when these metals are disr...

2026-03-18 | MTBLS13492 | MetaboLights
MetabolomicsgeoRge: A Computational Tool To Detect the Presence of Stable Isotope Labeling in LC/MS-Based Untargeted Metabolomics
Studying the flow of chemical moieties through the complex set of metabolic reactions that happen in the cell is essential to understanding the alterations in homeostasis that occur in disease. Recently, LC/MS-based untargeted metabolomics and isotopically labeled metabolites have been used to facil...
2015-09-15 | MTBLS213 | MetaboLights
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