HeLa cells (n=2) were treated with cell culture media containing 2 mM MGO or GO for 2 h and lysed in 6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, and 40 mM CAA. The lysates were dissolved by heating and sonication, followed by centrifugation at 20,000 x g for 15 min at 4 ºC. The superna...
ORGANISM(S): Homo Sapiens (human)
