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ProteomicsInteractome analysis for Gore-tex/Osiris23 and identification of ubiquitination sites in Gore-tex/Osiris23
To identify the proteins interacting with Gore-tex/Osiris23, affinity purification mass spectrometry (AP-MS) was performed using S2 cells transfected with HA or Flag-tagged Gore-tex/Osiris23 expression vectors. PTM analysis was also performed to determine the ubiquitination sites of Gore-tex/Osiris2...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2026-03-12 | PXD053779 | JPOST Repository
ProteomicsCmDnm1, in vitro phosphorylation by CmAUR, Gel
Recombinant Cyanidioschyzon Dnm1 protein (CmDnm1) was incubated with or without recombinant Cyanidioschyzon Aurora kinase (CmAUR). Proteins were separated by SDS-PAGE, in-gel tryptic digested, analyzed by LC-MS.
ORGANISM(S): Cellular Organisms 
2020-01-14 | PXD012329 | JPOST Repository
ProteomicsCmDnm1, in vitro phosphorylation by CmAUR
Recombinant Cyanidioschyzon Dnm1 protein (CmDnm1) was incubated with recombinant Cyanidioschyzon Aurora kinase (CmAUR). Proteins were tryptic digested and the phosphorylated peptides were concentrated with Titansphere Phos -TiO Kit. Finally, the enriched peptides were analyzed by LC-MS.
ORGANISM(S): Cyanidioschyzon Merolae 
2019-12-27 | PXD013717 | JPOST Repository
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