Proteins separated on 12%SDS gel, digested with trypsin after cutting the bands, the extracted Peptides were subjected to LC MS/MS on an Ion trap mass spectrometer (Finnigan LTQ from Thermo Electron Corporation).
Proteins separated on 12%SDS gel, digested with trypsin after cutting the bands, the extracted Peptides were subjected to LC MS/MS on an Ion trap mass spectrometer (Finnigan LTQ from Thermo Electron Corporation).
The proteins from Outer Membrane Vesicles (OMVs) were extracted by chilled acetone method. The proteins were separated on 1D SDS-PAGE (12%). From the gel, total 10 fractions were made and all of them were subjected to in gel digestion using trypsin. The MS/MS spectra of the resulting tryptic peptide...
Proteomic analysis of an Antarctic bacterium Pseudomonas sp. Lz4W by tandem mass spectrometry to unveil the process of cold adaptation. Comparative whole proteome analysis was performed at Low and optimum temperature.
Tryptic synthetic peptides generated in silico from human proteome are used as standards to evaluate the performance of MS and algorithm used to identify the proteins.
Outer membrane vesicles from A. baumannii DS002 were isolated using Diafilteration and Ultracentrifugation. The proteins of OMVs were separated on a 12.5% SDS-PAGE gel. The protein bands were excised and in-gel digetion was carried out using trypsin. The resultant peptides were analysed on LC-ESI-MS...
