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The process for making monocyte derived DCs (moDCs) has been previously described (46). All analysis was performed on “day 5” immature DCs. Etanercept 10mg/mL was added to experimental wells on days 0, 2, and 4. We chose this concentration of etanercept as it approximates the plasma concentra...
ORGANISM(S): Homo sapiens 
Protein Complexes Purification: Immunoprecipitations were performed with antibodies to LSD1 (2139; Cell signaling technology), INSM1 Mouse (SC-271408, Santa Cruz Biotechnology), RCOR2 (23969-1-AP; Proteintech Group), BRD9 (61537; Active Motif Rabbit), and normal Rabbit IgG (2729; Cell signaling tech...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-01-25 | MSV000083364 | MassIVE
Analysis of hybridisation performance and utility in identifying differentially expressed miRNAs of six miRNA microarray platforms using three biological samples in quadruplicate.
ORGANISM(S): Homo sapiens 
Mice were subjected to renal artery clamps to induce ischemia/reperfusion injury to the kidney. 3 days post surgery mice were injected with CSF1 or PBS daily for a further 3 days. Mice were sacrificed 5 and 7 days post surgey and the kidney was removed and homogenised into single cell suspensions. C...
ORGANISM(S): Mus Musculus 
Centrosome amplification is a common feature of human tumors, but whether this is a cause or a consequence of human cancer remains unclear. Here, we report the creation of a mouse model in which centrosome number can be persistently increased in the absence of additional genetic defects. We show tha...
ORGANISM(S): Mus musculus 
Bees from 3 unrelated colonies were injected with 1ul PBS extract containing 10^9 genome equivalent of Deformed wIng virus (DWV) and/or fed 10µl sucrose solution containing 10^5 fresh Nosema ceranae spores. Control bees were injected and fed with an equivalent DWV- and Nosema-free extract respectiv...
ORGANISM(S): Apis mellifera 
Transcriptional profiling of honey bee pupae challenging a DWV infection by comparing the gene expression profiles of untreated honey bee pupae with DWV-infected pupae. Goal was to determine the gene expression profile of DWV-infected honey bee pupae.
ORGANISM(S): Apis mellifera 

Hypodiploid acute lymphoblastic leukemia (ALL) is an aggressive form of leukemia characterized by multiple whole chromosomal losses and very poor outcome. Here we report an integrative genomic analysis that identifies multiple subtypes of hypodiploid ALL characterized by variation in the degree o...

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