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TranscriptomicsSingle-cell RNAseq of whole mouse E8.25 embryos
Gastrulation represents a pivotal point in mammalian development, when the basic body plan is established and cells are specified into one of the three germ layers. This is followed by rapid diversification into specific lineages and the appearance of the various cell types required to build each of...
ORGANISM(S): Mus musculus 
2017-12-01 | E-MTAB-5728 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of whole B6 mouse E8.25 embryos
Gastrulation represents a pivotal point in mammalian development, when the basic body plan is established and cells are specified into one of the three germ layers. This is followed by rapid diversification into specific lineages and the appearance of the various cell types required to build each of...
ORGANISM(S): Mus musculus 
2017-12-01 | E-MTAB-6153 | biostudies-arrayexpress
TranscriptomicsMouse embryonic RNA-seq
The study was aimed at interrogating the early stages of blood cell development within the embryo. Blood develops from mesoderm, which is specified at gastrulation. We therefore sorted cells from mouse embryos between E6.5 and E7.75 to capture gastrulation and the emergence of mesoderm, followed b...
ORGANISM(S): Mus Musculus 
2016-07-08 | E-MTAB-4079 | biostudies-arrayexpress
TranscriptomicsTimecourse single-cell RNAseq of whole mouse embryos harvested between days 6.5 and 8.5 of development.
We have captured 100,000 single cells for single-cell RNAseq from whole mouse embryos during gastrulation and organogenesis, spanning days 6.5 to 8.5 of development, including embryonic and extraembryonic tissues. Cells were sampled every six hours, providing a continuous molecular characterisation ...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-6967 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of control chimeric mouse embryos at embryonic days 7.5 and 8.5 of mouse development
Chimeric mouse embryos were generated as a control experiment to understand the contribution of host vs. injected cells to the developing embryo. Embryos were generated by blastocyst injection of tdTomato-labelled mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells wer...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-7324 | biostudies-arrayexpress
TranscriptomicsSingle-cell RNAseq of Tal1 knockout chimeric mouse embryos at embryonic day 8.5 of mouse development
Chimeric embryos were generated to investigate the effect of Tal1 knockout in mouse embryos by single-cell RNA-sequencing. Tal1 is an essential transcription factor for the formation of the embryonic blood. Embryo chimerism permits the analysis of the effects of Tal1 knockout without the confounding...
ORGANISM(S): Mus musculus 
2018-12-05 | E-MTAB-7325 | biostudies-arrayexpress
GenomicsSingle Cell Expression Profiling Resolves the Transcriptional Programs of Early Mesoderm Diversification
We analyzed 1,205 cells from the epiblast and nascent mesoderm of gastrulating mouse embryos by single cell RNA-seq, representing the first transcriptome-wide in vivo view of mammalian gastrulation. Based on the distribution of key marker genes and their spatiotemporal expression patterns within the...
ORGANISM(S): Mus musculus 
2016-07-07 | E-GEOD-74994 | biostudies-arrayexpress
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