Sort   by:  
 Page size 
UnknownSeptins stabilize mitochondria in Tetrahymena thermophila.
We describe phylogenetic and functional studies of three septins in the free-living ciliate Tetrahymena thermophila. Both deletion and overproduction of septins led to vacuolization of mitochondria, destabilization of the nuclear envelope, and increased autophagy. All three green fluorescent protein...
S-EPMC2519767 | biostudies-literature
UnknownHyperglutamylation of tubulin can either stabilize or destabilize microtubules in the same cell.
In most eukaryotic cells, tubulin is subjected to posttranslational glutamylation, a conserved modification of unclear function. The glutamyl side chains form as branches of the primary sequence glutamic acids in two biochemically distinct steps: initiation and elongation. The length of the glutamyl...
S-EPMC2805296 | biostudies-literature
UnknownMembers of the NIMA-related kinase family promote disassembly of cilia by multiple mechanisms.
The genome of Tetrahymena thermophila contains 39 loci encoding NIMA-related kinases (NRKs), an extraordinarily large number for a unicellular organism. Evolutionary analyses grouped these sequences into several subfamilies, some of which have orthologues in animals, whereas others are protist speci...
S-EPMC1474788 | biostudies-literature
UnknownKatanin regulates dynamics of microtubules and biogenesis of motile cilia.
The in vivo significance of microtubule severing and the mechanisms governing its spatial regulation are not well understood. In Tetrahymena, a cell type with elaborate microtubule arrays, we engineered null mutations in subunits of the microtubule-severing complex, katanin. We show that katanin act...
S-EPMC2064628 | biostudies-literature
UnknownDisAp-dependent striated fiber elongation is required to organize ciliary arrays.
Cilia-organizing basal bodies (BBs) are microtubule scaffolds that are visibly asymmetrical because they have attached auxiliary structures, such as striated fibers. In multiciliated cells, BB orientation aligns to ensure coherent ciliary beating, but the mechanisms that maintain BB orientation are ...
S-EPMC4274257 | biostudies-literature
UnknownGlutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena thermophila.
Tubulin undergoes glutamylation, a conserved posttranslational modification of poorly understood function. We show here that in the ciliate Tetrahymena, most of the microtubule arrays contain glutamylated tubulin. However, the length of the polyglutamyl side chain is spatially regulated, with the lo...
S-EPMC2519764 | biostudies-literature
UnknownFunctional study of genes essential for autogamy and nuclear reorganization in Paramecium.
Like all ciliates, Paramecium tetraurelia is a unicellular eukaryote that harbors two kinds of nuclei within its cytoplasm. At each sexual cycle, a new somatic macronucleus (MAC) develops from the germ line micronucleus (MIC) through a sequence of complex events, which includes meiosis, karyogamy, a...
S-EPMC3067474 | biostudies-literature
UnknownThe CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia.
Dynein motors and regulatory complexes repeat every 96 nm along the length of motile cilia. Each repeat contains three radial spokes, RS1, RS2, and RS3, which transduct signals between the central microtubules and dynein arms. Each radial spoke has a distinct structure, but little is known about the...
S-EPMC4395127 | biostudies-literature
Sort   by:  
 Page size