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ProteomicsReduction-Free Thermolysin Digestion Enables Absolute Protein Quantitation in Plasma in 35 min from Sample to Result
We previously reported that the broad-specificity protease thermolysin yields reproducible, near-complete proteome digests within 1–2 min. Here we demonstrate rapid absolute protein quantitation in human plasma by combining reduction/alkylation-free thermolysin digestion on S-Trap cartridges with fu...
ORGANISM(S): Homo Sapiens 
PXD077823 | panorama
ProteomicsPhosphoproteomics attributes aberrant signaling events to a druggable kinome in hepatocellular carcinom
To connect aberrant phospho-signaling events to therapeutic vulnerabilities in hepatocellular carcinoma (HCC), we performed a mass-spectrometry-based phosphoproteomic quantification of human HCC tumors, adjacent tissues and cell lines. We developed an integrated pipeline as the inference of druggabl...
ORGANISM(S): Homo Sapiens 
2023-01-02 | PXD023840 |
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