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TranscriptomicsGene expression profiling of murine T regulatory cells after insulin-like growth factor-1 stimulation
Treg cells were isolated from spleens of Foxp3cre/gfp mice, purified by FACS (CD4+ CD25+ GFP+), incubated with rhIGF-1 for two days, and further purified by FACS. RNA was prepared with the RNeasy Mini Kit according to the manufacturer’s instructions (Qiagen), followed by two rounds of RNA amplificat...
ORGANISM(S): Mus musculus 
2014-09-29 | E-MTAB-2951 | biostudies-arrayexpress
TranscriptomicsCoding mutations and loss-of-imprinting in human pluripotent cells derived by nuclear transfer and defined factors [DNA methylation profiling]
Human pluripotent stem cells can be derived from somatic cells by forced expression of defined factors, and more recently by nuclear-transfer into human oocytes, revitalizing a debate on whether one reprogramming approach might be advantageous over the other. Here we compared the genetic and epigene...
ORGANISM(S): Homo sapiens 
2014-11-06 | E-GEOD-61461 | biostudies-arrayexpress
TranscriptomicsCoding mutations and loss-of-imprinting in human pluripotent cells derived by nuclear transfer and defined factors [RNA-Seq]
Human pluripotent stem cells can be derived from somatic cells by forced expression of defined factors, and more recently by nuclear-transfer into human oocytes, revitalizing a debate on whether one reprogramming approach might be advantageous over the other. Here we compared the genetic and epigene...
ORGANISM(S): Homo sapiens 
2014-11-06 | E-GEOD-61656 | biostudies-arrayexpress
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