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TranscriptomicsThe dependence on NFAT levels discriminates conventional T cells from Foxp3 expressing regulatory cells
In this study, we used microarrays to investigate the gene expression program in conventional T cells, nTreg and conventional T cells treated with TGFbeta (iTreg) from wild-type mice and and mice having NFAT1/NFAT2 double-deficient (DKO) T cells. CD4+CD25- and CD4+CD25+ T cells were isolated by MACS...
ORGANISM(S): Mus musculus 
2012-10-15 | E-MEXP-3687 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of human regulatory and helper T cells following activation with anti-CD3 and anti-CD28 for 4 and 16 hours
Primary non-expanded human T cell populations were isolated by leukapheresis from 10 healthy donors. Out of each donor > 95% pure CD4-positive Th cell and >95% pure regulatory T cell populations were purified. Two affymetrix exon arrays were hybridized from each donor both populations as resting, no...
ORGANISM(S): Homo sapiens 
2010-05-18 | E-TABM-779 | biostudies-arrayexpress
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