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This experiment was designed to obtain the polyA+ transcriptome in E14 ESCs PolyA+ RNA was extracted and purified from two separate clones of E14, which were treated as biological replicate
ORGANISM(S): Mus musculus 
This experiment sought to determine the chromatin structure and PRC2 occupancy at the promoters of all genes in mouse ESCs ChIP-seq for EZH2, SUZ12, and H3K27me3 were performed on WT E14 cells in 2 biological replicates each. In addition H3K27me3 ChIP-seq was also performed on a clone of E14 cells e...
ORGANISM(S): Mus musculus 
This experiment was designed to indentify RNAs making direct contact JARID2 as mouse ESCs differentiate E14 WT were left untreated or differentiated with 2 M-BM-5M RA for 24 hrs. In both cases, ceslls were pulsed with 4-SU, irradiated with UV, and subjected to JARID2 immunoprecipitation.
ORGANISM(S): Mus musculus 
This experiment was designed to identify RNAs making direct contact with JARID2 in mouse embryonic stem cells E14 cells were pulsed with 4-SU, irradiated with UV, and subjected to JARID2 immunoprecipitation.
ORGANISM(S): Mus musculus 
This experiment was designed to indentify RNAs making direct contact with EZH2 in mouse embryonic stem cells E14 with an integrated transgene encoding HA-EZH2 were pulsed with 4-SU, irradiated with UV, and subjected to HA immunoprecipitation.
ORGANISM(S): Mus musculus 
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