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TranscriptomicsATAC-seq of HEK293 cells with Alu-motif targeted dCas9GFP
HEK293 cells were selected and subcloned for doxycyclin (DOX) and trimethoprim (TMP) inducible dCas9GFP and a guide RNA molecule targeting an EGA enriched Alu-motif (EEA-g1). Cells were treated with DOX and TMP for three days to induce dCas9GFP expression and ATAC-seq samples were collected from tre...
ORGANISM(S): Homo sapiens 
2018-06-07 | E-MTAB-6195 | biostudies-arrayexpress
TranscriptomicsSTRT RNAseq of pluripotent stem cells reprogrammed with CRISPRa
Transcriptome comparison of CRISPRa induced human pluripotent stem cell lines, control iPSC line induced with Sendai viral vectors, H9 embryonic stem cell line and human foreskin fibroblasts.
ORGANISM(S): Homo sapiens 
2018-06-07 | E-MTAB-6185 | biostudies-arrayexpress
TranscriptomicsDNA methylome analysis of human pluripotent stem cells reprogrammed with CRISPRa
Comparison of DNA methylation in CRISPRa induced human pluripotent stem cell lines, control iPSC line induced with Sendai viral vectors, H9 embryonic stem cell line and human foreskin fibroblasts.
ORGANISM(S): Homo sapiens 
2018-06-07 | E-MTAB-6194 | biostudies-arrayexpress
TranscriptomicsSTRT RNAseq of human foreskin fibroblasts undergoing CRISPRa reprogramming
Comparison of human foreskin fibroblasts with different guide RNA compositions undergoing CRISPRa-mediated reprogramming. Cell samples have been collected at days 4, 8 and 12 of pluripotency induction. Each time point has three conditions with either (1) pluripotent reprogramming factor promoter tar...
ORGANISM(S): Homo sapiens 
2018-06-07 | E-MTAB-6186 | biostudies-arrayexpress
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