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To understand how microRNAs are involved in stress response, we examined their expression changes in C. elegans animals that were exposed to stress conditions, including heat shock, oxidation, hypoxia and starvation. Total RNAs were purified from young adult animals that were exposed to each stress,...
ORGANISM(S): Caenorhabditis elegans 
We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries...
ORGANISM(S): Caenorhabditis elegans 
Recent work has shown that small non-coding RNAs, including miRNAs, serve an important role in controlling gene expression during development and disease. However, little detailed information exists concerning the relative expression patterns of small RNAs during development of C. elegans. Here we u...
ORGANISM(S): Caenorhabditis elegans 
To investigate the contribution of small non-coding RNAs, including microRNAs, to stress response in C. elegans, we examined their expression changes using Illumina deep-sequencing technology. Stress conditions we examined include heat shock, Pseudomonas aeruginosa (PA-14) and L1 arrest. For data u...
ORGANISM(S): Caenorhabditis elegans 
UMR106 cells were untreated or treated with 5 mM Pi or 100 ng/ml FGF2 for 15 min. The lysates were digested with trypsin, and tyrosine-phosphorylated peptides were enriched by anti-phosphotyrosine antibody. Several selected peptides including FGFR1 with phosphorylated tyrosine residues were measured...
ORGANISM(S): Rattus Rattus (black Rat) 
UMR106 cells were untreated or treated with 5 mM Pi or 100 ng/ml FGF2 for 15 min. The lysates were digested with trypsin, and tyrosine-phosphorylated peptides were enriched by anti-phosphotyrosine antibody and subjected to LC-MS/MS analysis. Raw data were directly analyzed against the SwissProt data...
ORGANISM(S): Rattus Rattus (black Rat) 
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