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UnknownAssociation of ITPA polymorphism with outcomes of peginterferon-α plus ribavirin combination therapy.
Not available
S-EPMC3729868 | biostudies-literature
ProteomicsLFQ of mNeonGreen-Trap IP-MS from cells expressing HKDC1-mNeonGreen
Cells stably expressing full-length HKDC1-mNeonGreen or △N20 HKDC1-mNeonGreen proteins were grown in a 10-cm dish and were treated with or without antimycin/oligomycin for 3h, and then cross-linked with 0.1% formaldehyde for 10min at room temperature. After cross-linking was quenched with 100mM glyc...
ORGANISM(S): Homo Sapiens (human) 
2024-01-21 | PXD040970 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-WIPI2d
Control or GFP-WIPI2d expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline co...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043701 | JPOST Repository
ProteomicsLFQ of GFP-Trap IP-MS from MEF cells expressing GFP-Rubicon
Control or GFP-Rubicon expressing MEFs were treated with or without 0.5uM Apilimod for one hour, and then cross-linked with 0.1% formaldehyde for ten mins at room temperature. After cross-linking was quenched with 100mM glycine for four mins at room temperature, cells were washed with HEPES saline c...
ORGANISM(S): Mus Musculus (mouse) 
2024-06-25 | PXD043695 | JPOST Repository
ProteomicsProximity biotinylation of NCI-H226 cells with gefitinib alone treatment by EGFR-FabID
NCI-H226 cells were pretreated with DMSO or gefitinib. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identifi...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD043527 | JPOST Repository
ProteomicsProximity biotinylation of A431 cells with gefitinib alone treatment by EGFR-FabID
A431cells were pretreated with DMSO or gefitinib. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD043526 | JPOST Repository
ProteomicsProximity biotinylation of NCI-H226 cells by EGFR-FabID
NCI-H226 cells were pretreated with DMSO or gefitinib and unstimulated or stimulated with EGF. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were ...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD039451 | JPOST Repository
ProteomicsProximity biotinylation of A431 cells by EGFR-FabID
A431 cells were pretreated with DMSO or gefitinib and unstimulated or stimulated with EGF. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were puri...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD039450 | JPOST Repository
ProteomicsProximity biotinylation of Expi293F cells stably expressing EGFR by EGFR-FabID
Expi293F cells stably expressing EGFR were treated with AGIA-FabID or EGFR-FabID in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis.
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD043525 | JPOST Repository
ProteomicsProximity biotinylation of Expi293F cells overexpressing EGFR by EGFR-FabID
Expi293F cells were transfected with pcDNA3.1-EGFR and treated with AGIA-FabID or EGFR-FabID in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS a...
ORGANISM(S): Homo Sapiens (human) 
2023-11-14 | PXD039449 | JPOST Repository
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