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ProteomicsImpact of Sample Preparation Strategies on The Quantitative Accuracy of Low-Abundance Serum Proteins in Shotgun Proteomics
Serum proteomics plays a crucial role in biomarker discovery and disease research, yet the selection of an optimal sample preparation method remains challenging. Evaluating the accuracy of protein quantitation is of major importance and a vital part of a benchmarking study in proteomics, since clini...
ORGANISM(S): Homo sapiens (Human) 
2025-09-15 | PXD062785 | Pride
Reproducibility Quantitative accuracy Serum proteomics Depth of coverage 
ProteomicsCross-Platform Evaluation Reveals Sample Preparation-Driven Biological Biases despite Advances in Mass Spectrometry Performance in Serum Proteomics
Advances in mass spectrometry (MS) instrumentation and protein extraction technologies are reshaping proteomics; however, whether improved analytical performance alters the biological information accessible from different sample preparation strategies remains unclear. Here, we performed a cross-plat...
ORGANISM(S): Homo sapiens (Human) 
2026-09-11 | PXD078563 | Pride
Serum proteomics Reproducibility Quantitative accuracy Depth of proteome Orbitrap exploris 480 Bruker tims-tof ht Biomarker discovery 
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