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TranscriptomicsMouse Sirt6-/- TNF-alpha timecourse
We completed genome-wide microarray analysis in Sirt6 knockout (ko) mouse embryonic fibroblasts (MEFs) and control wildtype (wt) MEFs. Prior to RNA extraction, MEFs were treated with TNF-alpha (10 ng/ml) for the indicated times. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Amb...
ORGANISM(S): Mus musculus 
2010-06-24 | E-GEOD-13207 | biostudies-arrayexpress
TranscriptomicsMouse Sirt6-/- tissues
We harvested liver, spleen, thymus, and skin tissues from 21 or 29 day old Sirt6-/- animals and then completed genome-wide microarray analysis. Total RNA was extracted with TRIzol (Invitrogen) and amplified with Amino Allyl MessageAmp II Amplification kit. Amplified age-matched wild-type tissues wer...
ORGANISM(S): Mus musculus 
2010-06-24 | E-GEOD-13208 | biostudies-arrayexpress
TranscriptomicsSirt6 study
This SuperSeries is composed of the following subset Series: GSE13206: Human shSIRT6 TNF-alpha timecourse GSE13207: Mouse Sirt6-/- TNF-alpha timecourse GSE13208: Mouse Sirt6-/- tissues GSE13209: Mouse Sirt6-/- RelA+/- tissues Refer to individual Series
ORGANISM(S): Mus musculus 
2010-06-24 | E-GEOD-13272 | biostudies-arrayexpress
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