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An rrf-1(pk1417) mutant strain was used to knockdown unc-32 gene function specifically in the germline, in which somatic RNAi, but not germline RNAi, was significantly repressed. Gravid adult hermaphrodites were collected in M9 buffer and bleached to obtain hard-shelled embryos. The embryos were was...
ORGANISM(S): Caenorhabditis Elegans 
Worms containing the transgene room-1::FLAG::GFP or room-2::FLAG::GFP were used for the Co-IP MS experiments. Approximately 25,000 gravid adult hermaphrodites were collected and homogenized in HBS buffer (20 mM HEPES-NaOH pH7.5, 150 mM NaCl, 1 mM MgCl2, and 1 mM EDTA with Complete Ultra Tablet Mini,...
ORGANISM(S): Caenorhabditis Elegans 
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