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Information regarding the prevalence of infectious agents in mice in pet shops in Japan is scarce. This information is particularly useful for minimizing the risk of potential transmission of infections to laboratory mice. Therefore, we surveyed infectious agents in mice from pet shops in Kanagawa a...
N-glycosylation of glycoproteins, a major post-translational modification, plays a crucial role in various biological phenomena. In central nervous systems, N-glycosylation is thought to be associated with differentiation and regeneration; however, the state and role of N-glycosylation in neuronal d...
Fc-receptors for immunoglobulin G (FcγRs) mediate a variety of effector and regulatory mechanisms in the immune system. N-glycosylation of FcγRs critically affects their functions which is well exemplified by antibody-dependent cell-mediated cytotoxicity (ADCC) and phagocytosis mediated by homologou...
Comprehensive membrane N-glycoproteomics using human breast cancer cell line pairs, Hs578T and Hs578Bst.
ORGANISM(S): Homo Sapiens (human) 
Human induced pluripotent stem cells were differentiated into NSC for 7 days. Cells on days 0, 1, 2, 3, 5 and 7 of differentiation (2.0 × 10⁶) were washed twice with DPBS (−) and then mixed with 100 μL of dissolution buffer consisting of 12 mM sodium deoxycholate, 12 mM N-lauroylsarcosine, 100 mM Tr...
ORGANISM(S): Homo Sapiens (human) 
The non-target siRNA transfected cells (Day 7) were washed twice with DPBS(-) and mixed in buffer D consisting of 12 mM sodium deoxycholate, 12 mM N-lauroylsarcosine, and 100 mM Tris-HCl pH 9.0. The cells were collected and incubated at 95 ℃ for 5 min. The samples were sonicated to remove viscous ma...
ORGANISM(S): Homo Sapiens (human) 
The cells (Days 0, 1, 2, 3, 5, 7, 10, 12, and 18) were washed twice with DPBS(-) and mixed in buffer D consisting of 12 mM sodium deoxycholate, 12 mM N-lauroylsarcosine, and 100 mM Tris-HCl pH 9.0. The cells were collected and incubated at 95 ℃ for 5 min. The samples were sonicated to remove viscous...
ORGANISM(S): Homo Sapiens (human) 
Clinical O-glycoproteomics for the development of diagnostic marker for colorectal cancer based on changes in O-glycosylation of serum protein.
ORGANISM(S): Homo Sapiens (human) 
Clinical O-glycoproteomics for the development of diagnostic marker for colorectal cancer based on changes in O-glycosylation of serum protein.
ORGANISM(S): Homo Sapiens (human) 
GATA6 is a transcription factor involved in the differentiation of intestinal epithelial cells into differentiated absorptive epithelial cells. GATA6 is expressed in all segments of the small intestine. We examined the impact of deleting GATA6 from intestinal epithelial cells of the adult ileum 6-8...
ORGANISM(S): Mus musculus 
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