The cells (Days 0, 1, 2, 3, 5, 7, 10, 12, and 18) were washed twice with DPBS(-) and mixed in buffer D consisting of 12 mM sodium deoxycholate, 12 mM N-lauroylsarcosine, and 100 mM Tris-HCl pH 9.0. The cells were collected and incubated at 95 ℃ for 5 min. The samples were sonicated to remove viscous...
ORGANISM(S): Homo Sapiens (human)