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ProteomicsSPANX immunoprecipitation analysis by mass spectrometry
A375 cells transduced with empty pLVX or pLVX-SPANXA-FLAG were subjected to immunoprecipitation using Flag beads. Elution from beads was performed by incubation with 3XFlag peptide for 1h at 4 C and eluates were subjected to tryptic digestion followed by LC-MS/MS, as described previously (PMID: 3099...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2019-11-24 | MSV000084617 | MassIVE
ProteomicsRNF5 interactome
Cells expressing Flag-tagged RNF5 or empty vector were incubated with the proteasome inhibitor MG132 to block protein degradation. Cell lysates were then prepared and immunoprecipitationed with anti-Flag antibody were performed, and eluates were digested with trypsin and analyzed on a Q Exactive.
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2018-11-25 | MSV000083160 | MassIVE
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