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TranscriptomicsHigh-throughput SuperSAGE for digital gene expression analysis of multiple samples using Next Generation Sequencing
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined “High-Throughput (HT-) SuperSAGE”. SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index...
ORGANISM(S): Danio rerio 
2010-09-30 | E-GEOD-20682 | biostudies-arrayexpress
TranscriptomicsThe time-series gene expression data in PMA stimulated THP-1
(1) qPCR Gene Expression Data The THP-1 cell line was sub-cloned and one clone (#5) was selected for its ability to differentiate relatively homogeneously in response to phorbol 12-myristate-13-acetate (PMA) (Sigma). THP-1.5 was used for all subsequent experiments. THP-1.5 cells were cultured in ...
ORGANISM(S): Homo sapiens 
2010-06-19 | E-GEOD-15528 | biostudies-arrayexpress
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