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To comparatively analyze the transcriptional responses of yeast cells to the presence of rapamycin or caffeine the homozygous hoΔ/hoΔ strain of S. cerevisiae BY4743 were grown in fully controlled fermenters in the presence of 200 nM rapamycin or 5 mM caffeine. Cells were cultured overnight in YPD me...
ORGANISM(S): Saccharomyces cerevisiae 
A 3 x 2 factorial design was used to elucidate the genome-wide transcriptional response to the deletion of yeast ortholog of Wilson and Menkes disease causing gene; CCC2, at changing copper levels. Homozygous deletion mutant of CCC2, which encodes Cu+2 transporting P-type ATPase required to export c...
ORGANISM(S): Saccharomyces cerevisiae 
Homozygous deletion mutants of drug resistance genes QDR3 and PDR3 and the homozygous deletion of HO (control) were grown in ammonium limited or glucose limited synthetic defined medium in aerated, pH controlled chemostats at a dilution rate of 0.1hr-1. Samples were collected at steady state.
ORGANISM(S): Saccharomyces cerevisiae 
We undertook an inter-laboratory effort to generate high-quality quantitative data for a very large number of cellular components in yeast using transcriptome and metabolome analysis. We ensured the high-quality of the experimental data by evaluating a wide range of sampling and measurement techniqu...
ORGANISM(S): Saccharomyces cerevisiae 
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