For human brain microvascular endothelial cells, we used adult passage 3 cortical cells (hBMVECs,
CellSystems, Kirkland, WA, USA). Cells were seeded at 13-15000 cells/cm2 in CSC complete
medium (Cell Systems, Kirkland, WA, USA) on tissue culture plastic culture dishes coated
with Attachment factor f...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)