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TranscriptomicsKlf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells
To characterize Klf4 downstream genes, we performed microarray analyses of the ES cells knocked down by siRNA of Klf4 gene. Keywords: individual genetic characteristic design ES cells grow without feeders on gelatin coated in complete ES medium. Cells were incubated at 37 0C; 5% CO2. ES cells were t...
ORGANISM(S): Mus musculus 
2010-06-10 | E-GEOD-5930 | biostudies-arrayexpress
TranscriptomicsDatabase for mRNA Half-Life of 19,977 Genes Obtained by DNA Microarray Analysis of Pluripotent and Differentiating Mouse
Degradation of mRNA is one of the key processes that control the steady-state level of gene expression. However, the rate of mRNA decay for the majority of genes is not known. We successfully obtained the rate of mRNA decay for 19 977 non-redundant genes by microarray analysis of RNA samples obtaine...
ORGANISM(S): Mus musculus 
2010-05-16 | E-GEOD-13609 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of global gene expression changes during mouse preimplantation development: unfertilized eggs, fertilized egg, 2-cell embryos, 4-cell embryos, 8-cell embryos, morulae, blastocysts
Microarray data analysis Intensity of 21,939 gene features per array was extracted from scanned microarray images using Feature Extraction 5.1.1 software (Agilent Technologies), which performs background subtractions and dye normalization. This normalization method is targeted at detecting changes i...
ORGANISM(S): Mus musculus 
2004-01-30 | E-MEXP-73 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice
Decreasing oocyte competence with maternal aging is a major factor in human infertility. To investigate the age-dependent molecular changes in a mouse model, we compared the expression profiles of metaphase II oocytes collected from 5-6 week old mice with those collected from 40-42 week old mice usi...
ORGANISM(S): Mus musculus 
2004-09-01 | E-MEXP-150 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse preimplantation development: unfertilized eggs, fertilized egg, 2-cell embryos, 4-cell embryos, 8-cell embryos, morulae, blastocysts
Feature Extraction 5.1.1 software (Agilent Technologies) performs background subtractions and dye normalization. This normalization method is targeted at detecting changes in relative expression of individual genes rather than global expression. Global expression change would require external normal...
ORGANISM(S): Mus musculus 
2004-01-12 | E-MEXP-74 | biostudies-arrayexpress
TranscriptomicsPluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells
To characterize the differentiation by Sox2 KO, we performed microarray analyses of mouse ES cell line 2TS22C during the time-course being induced of Sox2 KO Inducible Sox2 KO cell line 2TS22C were assigned to eight experimental groups and cultured in GMEM medium (SIGMA) with LIF at 37°C in an atmo...
ORGANISM(S): Mus musculus 
2010-06-11 | E-GEOD-5895 | biostudies-arrayexpress
TranscriptomicsInduction of cells expressing markers of GABAergic neurons by transcription factors
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Mus musculus 
2016-07-21 | E-GEOD-78951 | biostudies-arrayexpress
TranscriptomicsTimed induction of 10 transcription factors - ES time series data
To decipher the structure and behaviors of the transcription factor (TF) network, we created 50 permanent mouse ES cell lines, in each of which one of the 50 transcription factors tagged with FLAG, is inserted into the doxycycline (dox)-repressible ROSA26 locus. We have obtained time series data for...
ORGANISM(S): Mus musculus 
2010-05-23 | E-GEOD-16148 | biostudies-arrayexpress
GenomicsUncovering early response of gene regulatory networks in ES cells by systematic induction of transcription factors
This SuperSeries is composed of the following subset Series: GSE14559: Timed induction of 50 transcription factors in ES cells reveals a common mechanism to initiate differentiations GSE14586: Cdx2 Binding Sites On Cdx2 Expressing ES Cells GSE16148: Timed induction of 10 transcription factors - ES t...
ORGANISM(S): Mus musculus 
2009-09-30 | E-GEOD-16375 | biostudies-arrayexpress
GenomicsZscan4 mediates transient remodeling and transcriptional burst of heterochromatin in mouse embryonic stem cells
This SuperSeries is composed of the SubSeries listed below. Repetitive sequences such as telomeres, centromeres, and retrotransposons are packed in permanently-condensed and transcriptionally-silenced heterochromatin, whose maintenance is critical for the genome stability. We have recently found th...
ORGANISM(S): Mus musculus 
2015-09-10 | E-GEOD-51682 | biostudies-arrayexpress
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