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TranscriptomicsDeep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human Embryonic Stem cells
Cytoplasmic polyadenylated RNA from human embryonic stem cells (HES2) was separated on a 1.2% agarose and gel slices were excised corresponding to the following sizes: 0 to 0.5 kb; 0.5-2 kb; 2-3.5 kb; 3.5-6.5 kb and 6.5-20+ kb. Slices were dissolved and extracted RNA (1% of each fraction) was run on...
ORGANISM(S): Homo sapiens 
2010-10-20 | E-GEOD-24355 | biostudies-arrayexpress
TranscriptomicsDeep-transcriptome and ribonome sequencing redefines the molecular networks of pluripotency and the extracellular space in human Embryonic Stem cells (part 2)
Recent RNA-sequencing studies have shown remarkable complexity in the mammalian transcriptome. The ultimate impact of this complexity on the predicted proteomic output is less well defined. We have undertaken a strand-specific RNA sequencing of multiple cellular RNA fractions (>18Gb) to uncover the ...
ORGANISM(S): Homo sapiens 
2011-07-01 | E-GEOD-25842 | biostudies-arrayexpress
TranscriptomicsProfiling of FACs sorted subpopulations of iPS cells from cell surface marker profile
Two independent induced pluripotent stem cell lines: ES4CL1 (derived from foreskin) and MR90C2 (derived from lung Fibroblast) were maintained on inactivated mouse embryonic fibroblast (MEF) feeder cells in the presence of ESC media containing DMEM, 20% knock-out serum replacement with 100ng/mL of bF...
ORGANISM(S): Homo sapiens 
2013-06-07 | E-GEOD-15283 | biostudies-arrayexpress
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Not available
S-EPMC9487096 | biostudies-literature
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S-EPMC3652040 | biostudies-literature
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UnknownEstablishing the Convergent Validity of the Travel Habit Questions in the Health Behavior in School-Aged Children Questionnaire by Quantifying Active Travel in Norwegian Adolescents.
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UnknownComparison of three generations of ActiGraph activity monitors under free-living conditions: do they provide comparable assessments of overall physical activity in 9-year old children?
Not available
S-EPMC4100529 | biostudies-literature
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