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TranscriptomicsExpression data from E10.5 mouse otocyst
We established a novel EGFP reporter mouse line (named Tg(ETAR-EGFP)14Imeg), which enables the placode-derived inner ear sensory cell lineage to be visualized and monitored. At E10.5, EGFP expression was detected in the ventral and dorsomedial region of the otocyst. Using this reporter line and FACS...
ORGANISM(S): Mus musculus 
2011-05-23 | E-GEOD-19618 | biostudies-arrayexpress
ProteomicsLFQ_K5D1-cTEC_K5D1-beta5tKO-cTEC
Cortical TECs from K5D1 and K5D1-β5tKO mice were lysed, reduced, alkylated, and subjected to methanol/chloroform precipitation. Proteins were digested with trypsin/Lys-C mix. The digests were desalted using GL-Tip SDB, and the eluates were evaporated in a SpeedVac concentrator and dissolved in 0.1% ...
ORGANISM(S): Mus Musculus (mouse) 
2019-10-08 | PXD013133 | JPOST Repository
ProteomicsTMT10plex_cTEC_mTEC_beta5tKO-cTEC
TEC cells (K5D1 cTECs in quadruplicate, K5D1 mTECs in triplicate, and K5D1-β5tKO cTECs in triplicate) were lysed in 6 M guanidine-HCl containing 100 mM Tris-HCl, pH 8.0, and 2 mM DTT. The clarified lysates were reduced in 5 mM DTT and alkylated in 27.5 mM iodoacetamide. Proteins were purified by met...
ORGANISM(S): Mus Musculus (mouse) 
2019-10-08 | PXD013132 | JPOST Repository
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