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TranscriptomicsDetermining the wash solution to use
We tested a new wash solution from Agilent Keywords: Agilent microarray, pooling, Drosophila, intact adults Intact males and females for a single genotype were examined. Two independent replicates of each were compared on two slides in a dye flip.
ORGANISM(S): Drosophila melanogaster 
2010-06-10 | E-GEOD-5182 | biostudies-arrayexpress
TranscriptomicsDetermining the number of individuals to pool
We compared 10, 20 and 40 animal pools to determine the best number of intact adults to pool Keywords: Agilent microarray, pooling, Drosophila, intact adults 16 vials of flies were reared. From each vial 5 individuals were selected. For the 10 animal comparison 4 vials were used with each replicat...
ORGANISM(S): Drosophila melanogaster 
2010-06-10 | E-GEOD-5183 | biostudies-arrayexpress
TranscriptomicsSex-, stage-, and tissue-specific alternative splicing in Drosophila melanogaster
We used long-oligonucleotide microarrays to investigate whether alternative splicing in Drosophila is regulated in a sex-, stage-, or tissue-specific manner. To examine sex-specific splicing, we compared gene expression profiles of male and female pupae 12 hours after pupariation. To examine stage...
ORGANISM(S): Drosophila melanogaster 
2010-06-10 | E-GEOD-5190 | biostudies-arrayexpress
TranscriptomicsExpression data from adult male and female Drosophila melanogaster antenna, proboscis, and front leg
Sex-specific gene expression in sensory organs may play an important role in mating and foraging behavior. We used long-oligonucleotide microarrays to compare gene expression profiles of males and females in three adult appendages that carry large numbers of chemosensory organs – antenna, probosc...
ORGANISM(S): Drosophila melanogaster 
2010-06-10 | E-GEOD-5189 | biostudies-arrayexpress
TranscriptomicsQuantitative Genomics study of sex dimorphism in D. Melanogaster
A diallel including reciprocals but not selfs experiment. Keywords: Agilent microarray, sexual dimorphism, genetical genomics, genetic variation Nine randomly selected D. melanogaster lines were used as parents for this diallel. Male and female intact adult progeny were collected. Crosses were repe...
ORGANISM(S): Drosophila melanogaster 
2010-06-11 | E-GEOD-5181 | biostudies-arrayexpress
TranscriptomicsmRNA-Seq of whole adult females and males, and miixed sex embryos, from multiple Drosophila species
We performed transcriptional profiling of sexed whole adults and mixed sex embryos of eight Drosophila species. These data were used in a comparative transcriptomics analysis of multiple Drosophila species to define functional elements conserved throughout the Drosophila genus. For each species, wi...
ORGANISM(S): Drosophila rhopaloa 
2013-02-22 | E-GEOD-43321 | biostudies-arrayexpress
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