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ProteomicsHuman oocyte proteome and secretome
The proteome and secretome of human oocytes was studied by shotgun LCMSMS, starting from pools of 100 cells. Exploiting SP3, a novel technology for proteomic sample preparation using magnetic beads, we scaled down proteome analysis to single cells. Despite the low protein content of only ~100ng per...
ORGANISM(S): Homo sapiens (Human) 
2016-05-26 | PXD003691 | Pride
Human Oocytes Single-cell 
ProteomicsStudying the core circuitry of pluripotency by selective isolation of chromatin-associated proteins
Here we have developed a method to identify chromatin-bound partners of a protein of interest by selective isolation of chromatin-associated proteins (SICAP) followed by mass spectrometry. We applied SICAP to identify chromatin-binding proteins associated to Oct4, Sox2 and Nanog in mouse embryonic s...
ORGANISM(S): Mus musculus (Mouse) 
2016-12-19 | PXD003798 | Pride
Mass spectrometry Mouse embryonic stem cell Chromatin Sicap 
ProteomicsHuman oocyte proteome and secretome
The proteome and secretome of human oocytes was studied by shotgun LCMSMS, starting from pools of 100 cells. Exploiting SP3, a novel technology for proteomic sample preparation using magnetic beads, we scaled down proteome analysis to single cells. Despite the low protein content of only ~100ng per...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-11-15 | MSV000088387 | MassIVE
ProteomicsRBDmap - Comprehensive Identification of RNA-Binding Domains in Human Cells
The RNA interactomes of HeLa and HEK293 cells jointly comprise 1106 RNA-binding proteins (RBPs), with almost half of these lacking well-defined RNA-binding domains (RBDs), suggesting the existence of numerous unknown RNA-binding architectures. Here, we report on “RBDmap”, a new method built on inter...
ORGANISM(S): Homo sapiens (Human) 
2016-08-08 | PXD000883 | Pride
Rna binding domain Rna-binding proteins 
ProteomicsDrosophila mRNA Interactome - Global changes of the RNA-bound proteome during the maternal-to-zygotic transition in Drosophila
The maternal-to-zygotic transition (MZT) is a process that occurs in animal embryos at the earliest developmental stages, during which maternally deposited mRNAs and other molecules are degraded and replaced by products of the zygotic genome. The zygotic genome is not activated immediately upon fert...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2016-07-11 | PXD003882 | Pride
Tmt-ms3 Drosophila development Rna-binding proteins 
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2020-05-27 | PXD016576 | Pride
Streptavidin Affinity purification Mass spectrometry Dna pull down Chip sicap Lipid binding protein Bioid Membrane protein Chip Biotin 
ProteomicsProtease-resistant streptavidin for interaction proteomics
Streptavidin-mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners by mass spectrometry. Since contamination by streptavidin limits protein identification, we here present protease-resistant streptavidin beads (prS). Their application to diff...
ORGANISM(S): Mus musculus (Mouse) Homo sapiens (Human) 
2025-04-08 | PXD015858 | Pride
Lipid Mass spectrometry Interactors Streptavidin Membrane proteins Chip-sicap Biotinylation Bioid Chip Ceramide-binding proteins 
UnknownPleiotropic Effects of Metformin on the Chemotherapy Response of HPV-Positive Cancer Cells.
Not available
S-EPMC12169209 | biostudies-literature
Proteomicsnascent proteome analysis of HCT116 TP53 wild type and TP53 knockout cells treated with Neocarcinostatin (NCS) for 6h
In order to investigate the potential role of TP53 in regulating translation, HCT116 wild type and TP53 knockout cells were analyzed using both RNA sequencing, Ribosome sequencing and nascent proteome analysis. The cells were treated with 0.2 µg/ml Neocarcinostatin (NCS) to induce DNA damage and act...
ORGANISM(S): Homo sapiens (Human) 
2022-04-27 | PXD029512 | Pride
Tp53 Ncs Nascent proteome Metabolic labeling Dna damage 
ProteomicsAutomated sample preparation with SP3 for low-input clinical proteomics
High-throughput and streamlined workflows are essential in clinical proteomics for standardized processing of samples originating from a variety of sources, including frozen tissue, FFPE tissue, or blood. To reach this goal, we have implemented single-pot solid-phase-enhanced sample preparation (SP3...
ORGANISM(S): Homo sapiens (Human) 
2020-01-10 | PXD014556 | Pride
Sp3 Clinical proteomics Pulmonary adenocarcinoma Sample preparation Low-input High-throughput Automation 
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