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GenomicsDUX4 recruits p300/CBP through its C-terminus and induces global H3K27 acetylation changes
Ectopic expression of the double homeodomain transcription factor DUX4 causes facioscapulohumeral muscular dystrophy (FSHD). Mechanisms of action of DUX4 are currently unknown. Using immortalized human myoblasts with a titratable DUX4 transgene, we identify by mass spectrometry an interaction betwee...
ORGANISM(S): Homo sapiens 
2016-03-10 | E-GEOD-78158 | biostudies-arrayexpress
GenomicsEmbryonic stem cell based system for the discovery and mapping of developmental transcriptional programs
Chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) is the method of choice to study function of transcription factors in cell fate determination. This technique faces two major obstacles when applied to developmental studies: the availability of ChIP grade antibodies and access to ...
ORGANISM(S): Mus musculus 
2011-11-12 | E-GEOD-30882 | biostudies-arrayexpress
TranscriptomicsHoxA3 is an apical regulator of hemogenic endothelium
This SuperSeries is composed of the following subset Series: GSE25079: Epistasis analysis of Runx1 and Gata1 over HoxA3 in hemogenic endothelium GSE25080: Genes regulated by HoxA3 in endothelial and hematopoietic progenitors Refer to individual Series
ORGANISM(S): Mus musculus 
2010-12-20 | E-GEOD-25096 | biostudies-arrayexpress
TranscriptomicsNLRP7 promotes trophoblast lineage differentiation, YY1 binding and alters CpG methylation
Genome wide DNA methylation profiling of hESC in the untransduced, scrambled control and with knockdown of NLRP7 in undifferentiated and BMP4 differentiated states using the Illumina HumanMethylation 450 BeadChip. Several genomic sites exhibit altered methylation upon knocking down NLRP7. Bisulphite...
ORGANISM(S): Homo sapiens 
2013-11-04 | E-GEOD-45727 | biostudies-arrayexpress
TranscriptomicsA study of the differentiation of Murine ES cells [Scl+/hCD4 ES cells (Chung et al., 2002)] into hemangioblasts
Molecular mechanisms that regulate the generation of hematopoietic and endothelial cells from mesoderm are poorly understood. To define the underlying mechanisms, we compared gene expression profiles between embryonic stem (ES) cell-derived hemangioblasts (Blast-Colony-Forming Cells, BL-CFCs) and th...
ORGANISM(S): Mus musculus 
2007-01-25 | E-GEOD-6835 | biostudies-arrayexpress
TranscriptomicsGene expression analysis of inducible ES cells overexpressing Etv2 (induced for 12 hours at day 3 of differentiation)
During embryogenesis, the endothelial and the hematopoietic lineages first appear during gastrulation in the blood island of the yolk sac. We have previously reported that an Ets variant gene 2 (Etv2/ER71) mutant embryo lacks hematopoietic and endothelial lineages, however, the precise roles of Etv2...
ORGANISM(S): Mus musculus 
2012-07-03 | E-GEOD-37658 | biostudies-arrayexpress
TranscriptomicsRNA-seq of Mesp1-induced cardiopharyngeal mesoderm derivatives from ES cells
We have developed a protocol to generate cardiopharyngeal mesoderm (CPM) in vitro by Mesp1 induction in ES cells. The goal of this study is to compare the transcriptome of CPM-derived cardiac and skeletal myogenic progenitors to identify novel lineage-specific markers. mRNA profiles of CPM-derived ...
ORGANISM(S): Mus musculus 
2016-01-12 | E-GEOD-74682 | biostudies-arrayexpress
TranscriptomicsInducible Gata1 Suppression Expands Erythro-Megakaryocytic Progenitors from Embryonic Stem Cells
Embryonic stem (ES) and induced pluripotent stem (iPS) cells represent a potential source of megakaryocytes and platelets for transfusion therapies. However, most current ES/iPS cell differentiation protocols are limited by low yields of hematopoietic progeny. Mutations in the mouse and human genes ...
ORGANISM(S): Mus musculus 
2015-12-09 | E-GEOD-66479 | biostudies-arrayexpress
Transcriptomics662 adult acute myeloid leukemia (AML) cases characterized on the Affymetrix Human Genome U133 Plus 2.0 Microarray
The pretreatment karyotype of leukemic blasts is currently the key determinant in therapy decision-making in acute myeloid leukemia (AML). However, approximately fifty percent of AML patients, often carrying a normal karyotype, are currently unclassifiable based these established methods. Gene expre...
ORGANISM(S): Homo sapiens 
2016-06-23 | E-MTAB-3444 | biostudies-arrayexpress
TranscriptomicsExpression data from differentiating Flk1- and Flk1+ ES cells expressing Snail during Wnt inhibition
ES cells differentiated in the presence of the Wnt inhibitor DKK1 fail to express the transcription factor Snail and undergo EMT or mesoderm differentiation. We generated an ES cell line, A2.snail, that induced Snail expression upon addition of doxycycline addition. Microarrays were used to gain a ...
ORGANISM(S): Mus musculus 
2013-10-23 | E-GEOD-26524 | biostudies-arrayexpress
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