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TranscriptomicsWide-spread disruption of transcription termination in HSV-1 infection: Next-generation sequencing of translational activityd by ribosome profiling
Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Ribosome profiling was performed at various times during infection with minor modification to the protocol described in Stern-Ginossar N et al., Science...
ORGANISM(S): Homo sapiens 
2015-05-26 | E-GEOD-60040 | biostudies-arrayexpress
TranscriptomicsWide-spread disruption of transcription termination in HSV-1 infection: Next generation sequencing of total and newly transcribed (4sU-RNA) RNA
Primary human foreskin fibroblasts (HFF) were infected with wild-type simplex virus 1 (HSV-1) strain 17 at a multiplicity of infection (MOI) of 10. Newly transcribed RNA was labelled by adding 500µM 4-thiouridine (4sU) to the cell culture media for 1h. Total cellular RNA was isolated using Trizol. N...
ORGANISM(S): Homo sapiens 
2015-05-26 | E-GEOD-59717 | biostudies-arrayexpress
TranscriptomicsUltra short and progressive 4sU-tagging reveals key characteristics of RNA processing at nucleotide resolution
RNA synthesis and decay rates determine the steady-state levels of cellular RNAs. Metabolic tagging of newly transcribed RNA by 4-thiouridine (4sU) can reveal the relative contributions of RNA synthesis and decay rates. The kinetics of RNA processing, however, so far remained unresolved. Here, we sh...
ORGANISM(S): Homo sapiens 
2012-04-25 | E-GEOD-31653 | biostudies-arrayexpress
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