Here we describe the development of a robust method for RNA extraction and exome-capture RNA-sequencing of laser-capture microdissected (LCM) tumor cells (TC) and stromal immune cells (TIL) based on their morphology. We applied this method on seven tumor specimens and microbiopsies of triple-negativ...
We examined the performance and reproducibility of exome-capture RNA-seq with the TruSeq® protocol for gene expression profiling of microdissected TC (~20 mm2) FFPE and matched FF sections
