We sought to determine genes whose expression changed upon treatment with a selective inhibitor of class I PI3 kinase. Total of 6 samples: cells were treated with GDC-0941 at a concentration of 1mM for 6 hours (3 replicates) and control (3 replicates)
Frozen, whole tissue was dissociated and sorted into to tumor and stromal fractions via FACS Keywords: Mapping Array Profiling of tumor and stromal fractions from whole tissue
PURPOSE: Tailoring cancer treatment to tumor molecular characteristics promises to make personalized medicine a reality. However, reliable genetic profiling of archived clinical specimens has been hindered by limited sensitivity and high false positive rates. Here, we describe a novel methodology, M...
Breast cancer cell lines grown in full serum under standard conditions were profiled on Affymetrix GeneChip Mapping 100K Set Arrays Experiment Overall Design: Profiling of cell lines grown under standard conditions
