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TranscriptomicsRNA-seq of Col, h2a.w7, cmt3 and h2a.w7 cmt3 Arabidopsis thaliana floral buds
Floral buds at meiotic stage from Col, h2a.w7, cmt3 and h2a.w7 cmt3 Arabidopsis thaliana plants were extracted as previously described (Lambing at al, 2020 Plant Cell - PMID: 32024691), and cDNA used to produce libraries.
ORGANISM(S): Arabidopsis thaliana 
2025-07-13 | E-MTAB-14268 | biostudies-arrayexpress
GenomicsChIP-seq of ASY1 and controls on meiotic-stage floral buds of Arabidopsis
ChIP-seq of ASY1 was carried out on meiotic-stage floral buds of Arabidopsis using an a-ASY1 antibody. The experiment aims to determine the genome-wide profile of ASY1. ASY1 is a component of the chromosome axis and is expressed exclusively during meiosis. Two negative controls were used to test the...
ORGANISM(S): Arabidopsis thaliana 
2020-05-18 | E-MTAB-8705 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in Arabidopsis thaliana h2a.w7 Col-0 × Ler F2 populations using genotyping-by-sequencing
Genomic DNA from 144 h2a.w7 Col-0 x Ler F2 individuals was extracted using CTAB (as described in Kuo et al, 2022 Methods Mol Biol - PMID: 35461443) and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology - PMID: 31378616). Sequencing data was analysed to identify...
ORGANISM(S): Arabidopsis thaliana 
2025-07-11 | E-MTAB-15040 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in Arabidopsis thaliana wild type and asy1 Col-0 × Ws-4 F2 populations using genotyping-by-sequencing
Genomic DNA from 187 wild type and 169 asy1 Col-0 x Ws-4 F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan ...
ORGANISM(S): Arabidopsis thaliana 
2020-05-18 | E-MTAB-8715 | biostudies-arrayexpress
TranscriptomicsIdentifying crossover locations in Arabidopsis thaliana asy1/+ Col × Ler F2 populations using genotyping-by-sequencing
Genomic DNA from 191 asy1/+ Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Lawrence et al, 2019 Current Biology). Sequencing data was analysed to identify crossovers using the TIGER pipeline as previously described (Rowan et al, 2015 G3 (Bet...
ORGANISM(S): Arabidopsis thaliana 
2020-05-18 | E-MTAB-8725 | biostudies-arrayexpress
TranscriptomicsMeiotic cohesin, chromatin and expression profiling of Arabidopsis thaliana floral buds from wild type and non-CG DNA methylation/H3K9me2 pathway mutant plants (ChIP-seq)
We generated and sequenced ChIP libraries for the meiotic cohesin subunit REC8 and four histone modifications (H3K4me1, H3K4me2, H3K9me2 and H3K27me1) to investigate their relationships with meiotic chromosome architecture and recombination in Arabidopsis thaliana. REC8 and H3K9me2 ChIP-seq were per...
ORGANISM(S): Arabidopsis thaliana 
2020-01-21 | E-MTAB-7370 | biostudies-arrayexpress
TranscriptomicsMeiotic cohesin, chromatin and expression profiling of Arabidopsis thaliana floral buds from wild type and non-CG DNA methylation/H3K9me2 pathway mutant plants (RNA-seq)
To investigate the impact of disruption of the non-CG DNA methylation/H3K9me2 pathway upon transcription in Arabidopsis, we performed RNA-seq using meiotic-stage floral buds from wild type (Col-0) and kyp/suvh4 suvh5 suvh6 mutant plants. This enabled identification of differentially expressed genes ...
ORGANISM(S): Arabidopsis thaliana 
2020-01-21 | E-MTAB-7371 | biostudies-arrayexpress
UnknownArabidopsis HEAT SHOCK FACTOR BINDING PROTEIN is required to limit meiotic crossovers and HEI10 transcription
Not available
S-SCDT-10_15252-EMBJ_2021109958 | biostudies-other
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