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GenomicsGenome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding
We investigated the genome-wide binding sites for sigma54 and RNA polymerase in wild-type Escherichia coli MG1655.
ORGANISM(S): Escherichia coli 
2015-08-06 | E-MTAB-3697 | biostudies-arrayexpress
TranscriptomicsGenome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding
We investigated the effect that intragenic s54 binding sites have on transcripton elongation. In an rpoN deletion we have over-expressed rpoN from a plasmid (with vector only contol) and then looked at changes in transcription elongation around intragenic s54 binding sites by RNA-seq. We are also lo...
ORGANISM(S): Escherichia coli 
2015-08-06 | E-MTAB-3699 | biostudies-arrayexpress
TranscriptomicsChIP-seq of MG1655 wt, MG1655 ΔphoB, MG1655 Δhns, MG1655 ΔphoB+Δhns and MG1655 PhoB-FLAG strains grown in MOPS media with either low phosphate (0.2 mM) or high phosphate (1.32 mM)
We used ChIP-seq to map the binding of of C-terminally FLAG3-tagged PhoB to the Escherichia coli K-12 genome during growth in MOPS minimal media with low phosphate or high phosphate (0.2 mM or 1.32 mM, respectively). As a control, we performed ChIP-seq in an untagged strain. We also used ChIP-seq to...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2020-07-12 | E-MTAB-9293 | biostudies-arrayexpress
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