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TranscriptomicsExpression data from LCM captured prostate cancer cells
The prostate represents a complex mix of cell types and there is a need to analyze distinct cell populations to better understand their potential interactions. This study of cell-type specific gene expression patterns will contribute to understanding of how tumor epithelial cells may be affected by...
ORGANISM(S): Homo sapiens 
2010-04-10 | E-GEOD-20758 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of microdissected global and orbital layers of the rectus extraocular muscle (EOM) from adult Sprague-Dawley rats
Adult rat global and orbital layers of rectal extraocular muscles microdissected using Arcturus Pixcell II LCM are gene profiled here using affymetrix rat RAE230A gene chip. www.med.upenn.edu/cellbio/faculty/rubinstein/; www.uphs.upenn.edu/pmi/members/khurana/lab%20page.htm
ORGANISM(S): Rattus norvegicus 
2007-10-31 | E-GEOD-1374 | biostudies-arrayexpress
TranscriptomicsRNA-seq from tibialis muscles from rats aged 15 months (adult) and 27 months (old)
To gain new insights into molecular changes in skeletal muscle aging and disease with a special focus on differential alternative splicing and senescence, we performed RNA-seq on tibialis muscles from 15 months old (adult, a) and 27 months old (old, o) rats. Whole (w) muscle samples were prepared fo...
ORGANISM(S): Rattus norvegicus 
2021-06-26 | E-MTAB-10582 | biostudies-arrayexpress
TranscriptomicsSpatially distinct neutrophil responses within the inflammatory lesions of pneumonic plague
During pneumonic plague, the bacterium Yersinia pestis elicits the development of inflammatory lung lesions that continue to expand throughout infection. This lesion development and persistence is poorly understood. Here, we examine spatially distinct regions of lung lesions using laser capture micr...
ORGANISM(S): Mus musculus 
2015-07-13 | E-GEOD-70819 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of mouse detrusor, stroma and urothelium from neonatal bladder. (GUDMAP Series_id: 20)
The long term objective is to create an encyclopedia of the expression levels of all genes in multiple components of the developing bladder. The central thesis is straightforward. The combination of microdissected and laser capture microdissection (LCM) plus microarray analysis offers a powerful, e...
ORGANISM(S): Mus musculus 
2008-06-11 | E-GEOD-11221 | biostudies-arrayexpress
TranscriptomicsCombining laser microdissection and RNA-seq to chart the transcriptional landscape of fungal development
RNA-seq analysis of developmental stages from the filamenous ascomycete Sordaria macrospora. Using laser capture microdissection, we separated protoperithecia (young fruiting bodies) from the surrounding hyphae. RNA isolation and amplification from 150 protoperithecia yields enough material for RNA-...
ORGANISM(S): Sordaria macrospora 
2012-10-01 | E-GEOD-33668 | biostudies-arrayexpress
GenomicsLaser capture microscopy (LCM)-RNAseq for topological mapping of synovial pathology during rheumatoid arthritis
Laser capture microscopy (LCM)-RNAseq for topological mapping of synovial pathology during rheumatoid arthritis
PRJNA1087013 | ENA
GenomicsLaser capture microscopy (LCM)-RNAseq for topological mapping of synovial pathology during rheumatoid arthritis
Laser capture microscopy (LCM)-RNAseq for topological mapping of synovial pathology during rheumatoid arthritis
PRJNA1087014 | ENA
TranscriptomicsMolecular Characterization of Laser Microdissected Circumventricular Organs and Third Ventricle Ependyma in the Adult Rat by Microarray Analysis.
We used LCM and microarrays to analyze the transcriptomes of three CVOs, the SCO, the subfornical organ (SFO), and the PG, and the third ventricle ependyma in the adult rat in order to better characterize these organs at the molecular level. Gene expression was performed on laser microdissection of...
ORGANISM(S): Rattus norvegicus 
2012-05-23 | E-GEOD-35089 | biostudies-arrayexpress
TranscriptomicsLaser-capture microscopy coupled with Smart-seq2 (LCM-seq) for robust and efficient transcriptomic profiling of mouse and human tissues
Generally, when LCM is used in diverse transcriptomic analyses, several hundred, if not thousands, of cells are needed to obtain high quality of RNA-seq data. As some cellular populations are very small and tissue often in scarcity, we aimed to carefully document the lowest number of cells needed to...
ORGANISM(S): Homo sapiens 
2016-07-05 | E-GEOD-76514 | biostudies-arrayexpress
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